Detailed information of TA system TA227590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1155449..1156130
• Replicon: chromosome
• Accession: NZ_CP089064
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF35

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: LSP22_RS05505
• Protein ID: WP_003111825.1
• Coordinates: 1155765..1156130 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSP22_RS05500
• Protein ID: WP_003111824.1
• Coordinates: 1155449..1155772 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP22_RS05470 (LSP22_05470)	1150791..1151020	+	230	Protein_1083	helix-turn-helix transcriptional regulator	-
LSP22_RS05475 (LSP22_05475)	1151096..1151992	-	897	WP_124987557.1	hypothetical protein	-
LSP22_RS05480 (LSP22_05480)	1153233..1153814	+	582	WP_023105512.1	hypothetical protein	-
LSP22_RS05485 (LSP22_05485)	1153808..1154827	-	1020	Protein_1086	tyrosine-type recombinase/integrase	-
LSP22_RS05490 (LSP22_05490)	1154827..1155219	-	393	Protein_1087	hypothetical protein	-
LSP22_RS05495 (LSP22_05495)	1155214..1155333	+	120	Protein_1088	IS5/IS1182 family transposase	-
LSP22_RS05500 (LSP22_05500)	1155449..1155772	-	324	WP_003111824.1	XRE family transcriptional regulator	Antitoxin
LSP22_RS05505 (LSP22_05505)	1155765..1156130	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSP22_RS05510 (LSP22_05510)	1156394..1156711	-	318	WP_046602310.1	hypothetical protein	-
LSP22_RS05515 (LSP22_05515)	1156793..1157218	-	426	WP_003111827.1	VOC family protein	-
LSP22_RS05520 (LSP22_05520)	1157319..1158203	+	885	WP_003140892.1	LysR substrate-binding domain-containing protein	-
LSP22_RS05525 (LSP22_05525)	1158176..1159129	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
LSP22_RS05530 (LSP22_05530)	1159350..1159784	+	435	WP_003116494.1	RidA family protein	-
LSP22_RS05535 (LSP22_05535)	1159781..1161028	+	1248	WP_016561575.1	aminotransferase class V-fold PLP-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T227590 WP_003111825.1 NZ_CP089064:c1156130-1155765 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T227590 NZ_CP123000:c1357271-1357014 [Neorhizobium petrolearium]
ATGGAGATCACTTACGATGAACACAAGCGCCTGACCAATATCGAAAAGCACTTCGGCCTCGACTTTGCCGATCTCGATAT
GGATTTCTTCGAGGCCTCCACCATCATCCCCTCACGGGAGGATCGGCTTAAGGCCATCGGTGAATTCAGGGGCATGATCG
TCATCGCCGTGATCTTCACGCCGCTTGGCTCCGAAGCCATCTCCGTCATTTCAATGCGGCCGGCAAGCAGCAAGGAAAGG
AAAGTCCATGGCTACTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11887.72 Da
• Isoelectric Point: 5.8900

>AT227590 WP_003111824.1 NZ_CP089064:c1155772-1155449 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT227590 NZ_CP123000:c1357024-1356704 [Neorhizobium petrolearium]
ATGGCTACTAAGTTTTCATCGAAGAGGCCCCTCACCAAAGAGGAAGAGGCCGAAATCCAACGGATGATTGCATCCGACCC
GGATGCGCCGGAAGCGACCAATGAGGAGCTTGCGAAGGCGAAGCCGTTCCGGGAAGCCCTGCCCCATCTTGCCGCAAGTA
TCGACCGGGAGAGGGCTAGACGCGGGAGACCTCCGATTGAGAAGCCTCGCAAGCATGTATCTCTGCGTCTCGATCCGGAT
ATCATCGACCATTACAAGGCAACGGGCAAAGGCTGGCAGAGCCGAATGAACGAAGCGCTGCGCAAAGCCGCAGGCCTCTA
G

Structures

Toxin

• AlphaFold DB: V6AKP0