Detailed information of TA system TA227586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5217702..5218297
• Replicon: chromosome
• Accession: NZ_CP089063
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF37

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LSP18_RS24445
• Protein ID: WP_003113526.1
• Coordinates: 5218019..5218297 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSP18_RS24440
• Protein ID: WP_003099268.1
• Coordinates: 5217702..5218007 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP18_RS24395 (LSP18_24620)	5212761..5212943	-	183	WP_124185953.1	hypothetical protein	-
LSP18_RS24400 (LSP18_24625)	5212933..5213205	-	273	WP_004352675.1	hypothetical protein	-
LSP18_RS24405 (LSP18_24630)	5213317..5213589	+	273	WP_116806713.1	DNA-binding protein	-
LSP18_RS24410 (LSP18_24635)	5213645..5213980	+	336	WP_116806712.1	hypothetical protein	-
LSP18_RS24415 (LSP18_24640)	5213983..5214579	+	597	WP_147317308.1	hypothetical protein	-
LSP18_RS24420 (LSP18_24645)	5214576..5215067	+	492	WP_116806711.1	ImmA/IrrE family metallo-endopeptidase	-
LSP18_RS24425 (LSP18_24650)	5215074..5216201	+	1128	WP_116806710.1	beta family protein	-
LSP18_RS24430 (LSP18_24655)	5216198..5216848	-	651	WP_116806709.1	sce7726 family protein	-
LSP18_RS24435 (LSP18_24660)	5216955..5217365	-	411	WP_116806708.1	hypothetical protein	-
LSP18_RS24440 (LSP18_24665)	5217702..5218007	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
LSP18_RS24445 (LSP18_24670)	5218019..5218297	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSP18_RS24450 (LSP18_24675)	5218350..5218478	-	129	Protein_4840	integrase	-
LSP18_RS24455 (LSP18_24680)	5218626..5220854	+	2229	WP_043555265.1	TonB-dependent receptor	-
LSP18_RS24460 (LSP18_24685)	5220924..5221571	-	648	WP_003095021.1	carbonate dehydratase	-
LSP18_RS24465 (LSP18_24690)	5221633..5222871	-	1239	WP_057390779.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T227586 WP_003113526.1 NZ_CP089063:c5218297-5218019 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T227586 NZ_CP122997:c83034-82777 [Mycolicibacterium aubagnense]
GTGAGGAGCATCCACTTCGATCCCGACGCATGGGAGGACTTCCTGTTTTGGTTAGCCTCCGACCGCAAGATGGCCAGGCG
GATCACCAGACTCATCGGCGAAATCCAGCGCGACCCATTCGTCGGGATCGGCAAACCCGAACCCCTCAAGGGAGAACTAT
CCGGCTATTGGTCCCGCCGCATCGACGACGAGCACCGGCTCGTATACCGAGCAGACGACAACGAAGTCAAGATCATCAAA
GCTCGCTATCACTACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT227586 WP_003099268.1 NZ_CP089063:c5218007-5217702 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT227586 NZ_CP122997:c83318-83031 [Mycolicibacterium aubagnense]
ATGACCATCAGCGCAAGCGAAGCCCGACAACGACTCTTCCCACTCCTTGAACAGGTAAACACCGACCACGAACCTGTCCG
CATCGCGTCTAAAGCAGGCGATGCGGTACTTATGTCGGCCGACGACTACGACTCGTGGCAGGAGACCGTCTACCTGCTGC
GCTCGCCCGAGAATGCCCGCCGGCTCATGGAGGCCGTCGCCCGCGACAGGTCCGCCAGTGAGCCCACTCCGGCCGAATAC
GCCAAGACGATCGAAGAACTCGAGGAGATGGCCGGCGGATCCGCGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3