Detailed information of TA system TA227574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 143887..144482
• Replicon: chromosome
• Accession: NZ_CP089062
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF38

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LSP17_RS00670
• Protein ID: WP_003113526.1
• Coordinates: 144204..144482 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSP17_RS00665
• Protein ID: WP_003099268.1
• Coordinates: 143887..144192 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP17_RS00630 (LSP17_00630)	139036..139884	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
LSP17_RS00640 (LSP17_00640)	140051..140992	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
LSP17_RS00645 (LSP17_00645)	141109..141723	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
LSP17_RS00650 (LSP17_00650)	141765..142349	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
LSP17_RS00655 (LSP17_00655)	142390..143490	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
LSP17_RS00665 (LSP17_00665)	143887..144192	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
LSP17_RS00670 (LSP17_00670)	144204..144482	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSP17_RS00675 (LSP17_00675)	144535..144663	-	129	Protein_131	integrase	-
LSP17_RS00680 (LSP17_00680)	144811..147039	+	2229	WP_003099265.1	TonB-dependent receptor	-
LSP17_RS00685 (LSP17_00685)	147109..147756	-	648	WP_003095021.1	carbonate dehydratase	-
LSP17_RS00690 (LSP17_00690)	147818..149056	-	1239	WP_023119073.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T227574 WP_003113526.1 NZ_CP089062:c144482-144204 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T227574 NZ_CP122962:574103-574462 [Agrobacterium tumefaciens]
ATGGTCCGCAGTCAGGTTCCAAAACGTGGCGACGTTTATCTGGTCGACCTCAATCCTGTGATCGGCAGCGAAATCAGTGA
CGAACATCGTTGTATCGTCATCACGCCCAGAGAAATCAACGCGGTTGGACTGTGCCTTGTCGTTCCCGTGAACACGGGCG
GACTATTCACGCGCAGGGCGGGGCTTGCCGTGAACATATCGGGCCATAAGACAACCGGTGTCGCACTATGCAACCAGGTG
AGAAGCATGGACATTGTTGCCCGTGCCTCCCAGAAGAAAGCGAAATATATCGAAACCCTTGACGATGCTACGATCGACGA
AATTGTCGGTCGTGTCATCAGCATGATCGACCCCGCCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT227574 WP_003099268.1 NZ_CP089062:c144192-143887 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT227574 NZ_CP122962:573837-574103 [Agrobacterium tumefaciens]
ATGACCGTAACGACGAGAATAAGACGTCAGGGCGGTGCGGCGGTGATGACCATCCCGCCCGCTCTTTTGAAAATGCTGGG
ACTGGAAATCGGTGAGCAATTGACGCTGGAGGTCGATAACGGCGCGTTGGTCGCAAGCCCTGTTCGGCAGGAAAAGAAAC
GCTTCACGCTTGCCGAATTGCTGGAGGGGGCGGATGAAGTCGCCGCTCTCAATGCCAGCGCGCGGGAATGGGACGAAGCG
CCGCCAGTCGGCAAAGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: V6ALY3