Detailed information of TA system TA227570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1870012..1870607
• Replicon: chromosome
• Accession: NZ_CP089061
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: LSP19_RS08675
• Protein ID: WP_003113526.1
• Coordinates: 1870329..1870607 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSP19_RS08670
• Protein ID: WP_003099268.1
• Coordinates: 1870012..1870317 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP19_RS08650 (LSP19_08650)	1865367..1865639	-	273	WP_058197937.1	hypothetical protein	-
LSP19_RS08655 (LSP19_08655)	1865751..1866023	+	273	WP_058137854.1	hypothetical protein	-
LSP19_RS08660 (LSP19_08660)	1866183..1867952	+	1770	WP_079982741.1	DGQHR domain-containing protein	-
LSP19_RS08665 (LSP19_08665)	1867981..1869030	-	1050	WP_231786410.1	site-specific DNA-methyltransferase	-
LSP19_RS08670 (LSP19_08670)	1870012..1870317	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
LSP19_RS08675 (LSP19_08675)	1870329..1870607	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSP19_RS08680 (LSP19_08680)	1870660..1870788	-	129	Protein_1711	integrase	-
LSP19_RS08685 (LSP19_08685)	1870936..1873164	+	2229	WP_003099265.1	TonB-dependent receptor	-
LSP19_RS08690 (LSP19_08690)	1873234..1873881	-	648	WP_003095021.1	carbonate dehydratase	-
LSP19_RS08695 (LSP19_08695)	1873943..1875181	-	1239	WP_009316205.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T227570 WP_003113526.1 NZ_CP089061:c1870607-1870329 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T227570 NZ_CP122956:1404530-1404811 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTACAGAAAGCGA
TGTTACGAGCGTTGTTGATCGCCTCAAAGAGCAAAGACCACTCCAACAAAAATATTGCGACCATGCCTTAAGTGGCAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT227570 WP_003099268.1 NZ_CP089061:c1870317-1870012 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT227570 NZ_CP122956:1404223-1404537 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3