Detailed information of TA system TA227567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 50473..51124
• Replicon: plasmid pRIVM_C010559_2
• Accession: NZ_CP089053
• Organism: Acinetobacter ursingii strain RIVM_C010559

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: LSO60_RS17980
• Protein ID: WP_004668307.1
• Coordinates: 50473..50829 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSO60_RS17985
• Protein ID: WP_001140620.1
• Coordinates: 50822..51124 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSO60_RS17955 (LSO60_17915)	45612..46315	+	704	Protein_40	IS6-like element IS1006 family transposase	-
LSO60_RS17960 (LSO60_17920)	46366..46799	+	434	Protein_41	recombinase family protein	-
LSO60_RS17965 (LSO60_17925)	47025..47375	-	351	WP_004644745.1	DUF3147 family protein	-
LSO60_RS17970 (LSO60_17930)	47566..48492	+	927	WP_005098232.1	chromate resistance protein	-
LSO60_RS17975 (LSO60_17935)	48489..49838	+	1350	WP_005098231.1	chromate efflux transporter	-
LSO60_RS17980 (LSO60_17940)	50473..50829	+	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSO60_RS17985 (LSO60_17945)	50822..51124	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
LSO60_RS17990 (LSO60_17950)	51166..51327	-	162	Protein_47	IS6 family transposase	-
LSO60_RS17995 (LSO60_17955)	51421..51813	-	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
LSO60_RS18000 (LSO60_17960)	51903..53183	+	1281	WP_005093338.1	cation transporter	-
LSO60_RS18005 (LSO60_17965)	53339..54271	+	933	WP_263513449.1	IS5-like element IS17 family transposase	-
LSO60_RS18010 (LSO60_17970)	54360..55447	+	1088	Protein_51	IS3-like element ISAba22 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..211456	211456
	-	inside	IScluster/Tn	-	-	45612..59620	14008

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T227567 WP_004668307.1 NZ_CP089053:50473-50829 [Acinetobacter ursingii]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T227567 NZ_CP122955:c1095242-1094982 [Helicobacter pylori]
ATGCTGAAAATTAGGCATCATATCTTTTTCAAAAAAGACTTTAAAAAGCTTGTTAAAAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAAGAAAAAAAGAACCGCTAGATCCACAATTTCAAGATCATGCCCTAAAAGGCAAGTGGAAACCTT
TTAGGGAATGCCACATCAAAGCTGATGTTTTGCTTGTGTATTTAGTGCGAGATGATGAGCTTGTTTTGTTAAGGTTAGGC
AGTCATAGCGAGCTGTTTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT227567 WP_001140620.1 NZ_CP089053:50822-51124 [Acinetobacter ursingii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT227567 NZ_CP122955:c1095600-1095229 [Helicobacter pylori]
ATGCCTAATTCTCCCACCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCA
AAAAATCAAAAAGATGCAAAATGATAGAGCTTCTTTCAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGGACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGTTAAATGGCGAAAACTTAGAAACTATTGAAGA
CTTTTCTGCATGGGCAAATGAAGTGAAAAAGGAAATGAATGCTGAAAATTAG

Structures

Toxin

• AlphaFold DB: N9NM16