Detailed information of TA system TA227565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 258744..259329
• Replicon: plasmid pRIVM_C010559_1
• Accession: NZ_CP089052
• Organism: Acinetobacter ursingii strain RIVM_C010559

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: LSO60_RS17470
• Protein ID: WP_000897307.1
• Coordinates: 258744..259064 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSO60_RS17475
• Protein ID: WP_000369781.1
• Coordinates: 259057..259329 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSO60_RS17440 (LSO60_17400)	254288..254497	-	210	WP_004976362.1	hypothetical protein	-
LSO60_RS17445 (LSO60_17405)	254749..255051	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LSO60_RS17450 (LSO60_17410)	255052..255333	+	282	WP_000985609.1	putative addiction module antidote protein	-
LSO60_RS17455 (LSO60_17415)	255413..255862	-	450	WP_228719708.1	SMI1/KNR4 family protein	-
LSO60_RS17460 (LSO60_17420)	255897..257028	+	1132	Protein_249	IS3-like element ISAba14 family transposase	-
LSO60_RS17465 (LSO60_17425)	257295..258619	+	1325	Protein_250	ISNCY family transposase	-
LSO60_RS17470 (LSO60_17430)	258744..259064	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSO60_RS17475 (LSO60_17435)	259057..259329	+	273	WP_000369781.1	NadS family protein	Antitoxin
LSO60_RS17480 (LSO60_17440)	259822..261297	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
LSO60_RS17485 (LSO60_17445)	261353..262237	+	885	WP_171265707.1	Mph(E) family macrolide 2'-phosphotransferase	-
LSO60_RS17490 (LSO60_17450)	262428..263039	-	612	WP_015060246.1	recombinase family protein	-
LSO60_RS17495 (LSO60_17455)	263295..264257	+	963	WP_033917576.1	IS30-like element IS18 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / blaCARB-2 / sul1 / dfrA19 / blaIMP-4 / catB3 / ARR-3 / qacE / aph(3')-VIa / msr(E) / mph(E) / blaOXA-58 / aac(3)-IId	-	1..313387	313387
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	250099..264257	14158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T227565 WP_000897307.1 NZ_CP089052:258744-259064 [Acinetobacter ursingii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T227565 NZ_CP122952:c1000307-1000026 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATTTTATCTGGGCGTATTACAGAAAACGA
TGTTACAAGTATTGTTGATTGCCTAAAAAAACAAAAACCACTCCAACAAAAATATTGCGACCATGCTTTGAGTGGCAATC
TTAAAGGGTTGAGAGAATGCCATGTCAAACCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT227565 WP_000369781.1 NZ_CP089052:259057-259329 [Acinetobacter ursingii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT227565 NZ_CP122952:c1000614-1000300 [Helicobacter pylori]
ATGTCAAATGTTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAAGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAGG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACCAAGTTTGTTAAATACGATCGCTTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTGCAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3