Detailed information of TA system TA227559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 150123..150708
• Replicon: plasmid pRIVM_C010761_1
• Accession: NZ_CP089045
• Organism: Acinetobacter ursingii strain RIVM_C010761

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: LSO58_RS17220
• Protein ID: WP_000897307.1
• Coordinates: 150388..150708 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSO58_RS17215
• Protein ID: WP_000369781.1
• Coordinates: 150123..150395 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSO58_RS17195 (LSO58_17165)	145195..146157	-	963	WP_033917576.1	IS30-like element IS18 family transposase	-
LSO58_RS17200 (LSO58_17170)	146413..147024	+	612	WP_015060246.1	recombinase family protein	-
LSO58_RS17205 (LSO58_17175)	147215..148099	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
LSO58_RS17210 (LSO58_17180)	148155..149630	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
LSO58_RS17215 (LSO58_17185)	150123..150395	-	273	WP_000369781.1	NadS family protein	Antitoxin
LSO58_RS17220 (LSO58_17190)	150388..150708	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSO58_RS17225 (LSO58_17195)	150938..152082	-	1145	Protein_129	IS3 family transposase	-
LSO58_RS17230 (LSO58_17200)	152160..152871	+	712	Protein_130	IS66 family transposase	-
LSO58_RS17235 (LSO58_17210)	152834..153298	+	465	WP_263503854.1	SAVED domain-containing protein	-
LSO58_RS17240 (LSO58_17215)	153849..154771	+	923	Protein_132	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58 / aac(3)-IId / mph(E) / msr(E) / sul1 / qacE / catB3 / blaIMP-4 / dfrA19 / blaCARB-2 / aph(3')-Ia	-	1..258901	258901
	-	inside	IScluster/Tn	mph(E) / msr(E)	-	145195..154538	9343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T227559 WP_000897307.1 NZ_CP089045:c150708-150388 [Acinetobacter ursingii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T227559 NZ_CP122948:606889-607146 [Helicobacter pylori]
ATGCTGATGATTGAAACCAGTAAAAAATTTGATAAGGATCTTAAAATTCTTGTTAAAAACGGGTTTGATTTAAAGCTTTT
ATATAAAGTGGTTGGAAATTTAGCCAAAGAGCAACCCCTAGCTTCCAAATACAAAGACCACCCGCTCAAGGGTGCTTTAA
AAGATTTTAGGGAATGCCACCTAAAACCGGATTTATTGCTTGTCTATCAAATTAAAAAACAAGAAAACACCCTCTTTTTA
TTGACATACTCCCCATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT227559 WP_000369781.1 NZ_CP089045:c150395-150123 [Acinetobacter ursingii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT227559 NZ_CP122948:606594-606902 [Helicobacter pylori]
ATGCCAAACACCACCAACAAAGATTACACAAAATACAGCCAAAGACAGCTTTTTAATTTTTTAAATTCTATCAAGACCAA
ACAAAAAAGAGCGTTAGAAAAATTGAAAGAAATCCAAGCTCAAAAACAAAGAATCAAAAAAGCGCTCCAATTTAAAGCGC
TCCATTTAACCGAAAACGGATACACCATAGAAGAAGAGCGAGAGATCTTAGCAAGAGCTAAAGACACCAAGAACCACCTT
TGTTTTAAAAATATAGAGGATTTTAAAAAGCATTGTGAAAACTTAAGATTTAAAAATGCTGATGATTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3