Detailed information of TA system TA227553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 232765..233350
• Replicon: plasmid pRIVM_C011601_1
• Accession: NZ_CP089039
• Organism: Acinetobacter ursingii strain RIVM_C011601

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: LSO59_RS18070
• Protein ID: WP_000897307.1
• Coordinates: 232765..233085 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSO59_RS18075
• Protein ID: WP_000369781.1
• Coordinates: 233078..233350 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSO59_RS18045 (LSO59_18015)	227897..228295	-	399	WP_228719673.1	SAVED domain-containing protein	-
LSO59_RS18050 (LSO59_18020)	228258..228479	-	222	Protein_217	transposase domain-containing protein	-
LSO59_RS18055 (LSO59_18025)	228584..230245	-	1662	WP_005167203.1	group II intron reverse transcriptase/maturase	-
LSO59_RS18060 (LSO59_18030)	230822..231313	-	492	Protein_219	IS66 family transposase	-
LSO59_RS18065 (LSO59_18035)	231403..232535	+	1133	WP_223155595.1	IS3-like element ISAba14 family transposase	-
LSO59_RS18070 (LSO59_18040)	232765..233085	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSO59_RS18075 (LSO59_18045)	233078..233350	+	273	WP_000369781.1	NadS family protein	Antitoxin
LSO59_RS18080 (LSO59_18050)	233843..235318	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
LSO59_RS18085 (LSO59_18055)	235374..236258	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
LSO59_RS18090 (LSO59_18060)	236449..237060	-	612	WP_015060246.1	recombinase family protein	-
LSO59_RS18095 (LSO59_18065)	237316..238278	+	963	WP_033917576.1	IS30-like element IS18 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / blaCARB-2 / qacE / sul1 / dfrA19 / blaIMP-4 / catB3 / ARR-3 / msr(E) / mph(E) / aac(3)-IId / blaOXA-58	-	1..317317	317317
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	226346..238278	11932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T227553 WP_000897307.1 NZ_CP089039:232765-233085 [Acinetobacter ursingii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T227553 NZ_CP122943:36473-36751 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGACGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT227553 WP_000369781.1 NZ_CP089039:233078-233350 [Acinetobacter ursingii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT227553 NZ_CP122943:36204-36473 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCACGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3