Detailed information of TA system TA227545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4427957..4428651
• Replicon: chromosome
• Accession: NZ_CP089036
• Organism: Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: CO544_RS22555
• Protein ID: WP_001263495.1
• Coordinates: 4427957..4428355 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CO544_RS22560
• Protein ID: WP_000554757.1
• Coordinates: 4428358..4428651 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4423544)	4423544..4423624	-	81	NuclAT_11	-	-
- (4423544)	4423544..4423624	-	81	NuclAT_11	-	-
- (4423544)	4423544..4423624	-	81	NuclAT_11	-	-
- (4423544)	4423544..4423624	-	81	NuclAT_11	-	-
CO544_RS22530 (4424220)	4424220..4424678	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CO544_RS22535 (4424939)	4424939..4426396	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CO544_RS22540 (4426453)	4426453..4426974	-	522	Protein_4425	peptide chain release factor H	-
CO544_RS22545 (4426973)	4426973..4427176	-	204	Protein_4426	RtcB family protein	-
CO544_RS22550 (4427495)	4427495..4427947	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
CO544_RS22555 (4427957)	4427957..4428355	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CO544_RS22560 (4428358)	4428358..4428651	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CO544_RS22565 (4428703)	4428703..4429758	-	1056	WP_001226186.1	DNA polymerase IV	-
CO544_RS22570 (4429829)	4429829..4430614	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CO544_RS22575 (4430586)	4430586..4432298	+	1713	Protein_4432	flagellar biosynthesis protein FlhA	-
CO544_RS22580 (4432443)	4432443..4432940	-	498	Protein_4433	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T227545 WP_001263495.1 NZ_CP089036:c4428355-4427957 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T227545 NZ_CP122938:c3991118-3990198 [Escherichia coli]
ATGGATCTGCGTGATAGTTATGCCCGGCAGGTTAAGCTGTTGATGGCTGCTCTGCCGCATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACGGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACTGATCTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCCCCAAACTGGGATATAGAGCGGATCACGACGCTGTATGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGCGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT227545 WP_000554757.1 NZ_CP089036:c4428651-4428358 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT227545 NZ_CP122938:c3991887-3991105 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAATACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAAAATGGCTGGCTCAGGCGCATCGCTCACGGCGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTGCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTACAGGGCCATGCGCACTATACCGAACCGGGCAGGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTTAA
GCTGCCGGGATGGTTTGCTGCCTTCGAACAATCTGCAACATTTATCACGCTGTATACATCGGGGCTTACCGTAGATGTAA
CGTCATTGACTACGGAACTAAAAATTGGCGACTGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6