Detailed information of TA system TA227540

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3541342..3541567
• Replicon: chromosome
• Accession: NZ_CP089036
• Organism: Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: CO544_RS18405
• Protein ID: WP_000813263.1
• Coordinates: 3541342..3541497 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3541509..3541567 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO544_RS18370	3536796..3537509	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
CO544_RS18375	3537647..3537843	-	197	Protein_3608	TrmB family transcriptional regulator	-
CO544_RS18380	3538130..3538948	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
CO544_RS18385	3539100..3539471	-	372	WP_000090264.1	antiterminator Q family protein	-
CO544_RS18390	3539461..3539832	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
CO544_RS18395	3539845..3540894	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
CO544_RS18400	3540896..3541174	-	279	WP_001341388.1	hypothetical protein	-
CO544_RS18405	3541342..3541497	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3541509..3541567	+	59	-	-	Antitoxin
CO544_RS18415	3542102..3542875	+	774	WP_000160654.1	alpha/beta hydrolase	-
CO544_RS18420	3543227..3543640	-	414	WP_001151233.1	DUF977 family protein	-
CO544_RS18425	3543656..3544426	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
CO544_RS18430	3544448..3545194	-	747	WP_000788745.1	ATP-binding protein	-
CO544_RS18435	3545201..3546298	-	1098	WP_001475117.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T227540 WP_000813263.1 NZ_CP089036:c3541497-3541342 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T227540 NZ_CP122938:2843510-2843617 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTTGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT227540 NZ_CP089036:3541509-3541567 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin