Detailed information of TA system TA227536

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2677453..2677678
• Replicon: chromosome
• Accession: NZ_CP089036
• Organism: Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: CO544_RS13780
• Protein ID: WP_000813258.1
• Coordinates: 2677523..2677678 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2677453..2677511 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO544_RS13730	2672518..2672760	+	243	WP_000747948.1	Cro/CI family transcriptional regulator	-
CO544_RS13735	2672744..2673169	+	426	WP_000693878.1	toxin YdaT family protein	-
CO544_RS13740	2673238..2674281	+	1044	WP_001262402.1	DnaT-like ssDNA-binding domain-containing protein	-
CO544_RS13745	2674274..2674735	+	462	WP_000139447.1	replication protein P	-
CO544_RS13750	2674769..2675485	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
CO544_RS13755	2675518..2675799	+	282	WP_000603384.1	DNA-binding protein	-
CO544_RS13760	2675796..2676023	+	228	WP_000699809.1	hypothetical protein	-
CO544_RS13765	2676016..2676327	+	312	WP_001289673.1	hypothetical protein	-
CO544_RS13770	2676455..2676673	+	219	WP_000683609.1	DUF4014 family protein	-
CO544_RS13775	2676675..2677232	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2677453..2677511	-	59	-	-	Antitoxin
CO544_RS13780	2677523..2677678	+	156	WP_000813258.1	Hok/Gef family protein	Toxin
CO544_RS13785	2677798..2678142	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
CO544_RS13790	2678264..2678536	+	273	WP_000191872.1	hypothetical protein	-
CO544_RS13795	2678538..2679587	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
CO544_RS13800	2679600..2679905	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
CO544_RS13805	2679968..2680522	+	555	WP_000640035.1	DUF1133 family protein	-
CO544_RS13810	2680747..2680944	+	198	WP_000917763.1	hypothetical protein	-
CO544_RS13815	2681080..2681793	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
CO544_RS13830	2682244..2682675	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2609071..2709914	100843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T227536 WP_000813258.1 NZ_CP089036:2677523-2677678 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T227536 NZ_CP122938:2092816-2092919 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 59 bp

>AT227536 NZ_CP089036:c2677511-2677453 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin