Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 745386..746113 Replicon chromosome
Accession NZ_CP089036
Organism Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag CO544_RS03680 Protein ID WP_000550189.1
Coordinates 745386..745700 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CO544_RS03685 Protein ID WP_000560249.1
Coordinates 745697..746113 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO544_RS03660 (741544) 741544..742530 - 987 WP_000617675.1 Gfo/Idh/MocA family oxidoreductase -
CO544_RS03665 (742609) 742609..743301 - 693 WP_000942543.1 vancomycin high temperature exclusion protein -
CO544_RS03670 (743378) 743378..743881 - 504 WP_001295542.1 M48 family metallopeptidase -
CO544_RS03675 (743966) 743966..745102 + 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
CO544_RS03680 (745386) 745386..745700 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
CO544_RS03685 (745697) 745697..746113 + 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
CO544_RS03690 (746158) 746158..748176 - 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
CO544_RS03695 (748702) 748702..751053 - 2352 WP_000695517.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T227524 WP_000550189.1 NZ_CP089036:745386-745700 [Escherichia coli O157:H7]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T227524 NZ_CP122929:3714094-3714372 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT227524 WP_000560249.1 NZ_CP089036:745697-746113 [Escherichia coli O157:H7]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT227524 NZ_CP122929:3713831-3714091 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References