Detailed information of TA system TA227511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4613867..4614561
• Replicon: chromosome
• Accession: NZ_CP089034
• Organism: Escherichia coli O157:H7 strain 6535NalR

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: LSI70_RS23760
• Protein ID: WP_001263495.1
• Coordinates: 4613867..4614265 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LSI70_RS23765
• Protein ID: WP_000554757.1
• Coordinates: 4614268..4614561 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4609454)	4609454..4609534	-	81	NuclAT_12	-	-
- (4609454)	4609454..4609534	-	81	NuclAT_12	-	-
- (4609454)	4609454..4609534	-	81	NuclAT_12	-	-
- (4609454)	4609454..4609534	-	81	NuclAT_12	-	-
LSI70_RS23735 (4610130)	4610130..4610588	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LSI70_RS23740 (4610849)	4610849..4612306	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
LSI70_RS23745 (4612363)	4612363..4612884	-	522	Protein_4663	peptide chain release factor H	-
LSI70_RS23750 (4612883)	4612883..4613086	-	204	Protein_4664	RtcB family protein	-
LSI70_RS23755 (4613405)	4613405..4613857	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
LSI70_RS23760 (4613867)	4613867..4614265	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LSI70_RS23765 (4614268)	4614268..4614561	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LSI70_RS23770 (4614613)	4614613..4615668	-	1056	WP_001226188.1	DNA polymerase IV	-
LSI70_RS23775 (4615739)	4615739..4616524	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
LSI70_RS23780 (4616496)	4616496..4618208	+	1713	Protein_4670	flagellar biosynthesis protein FlhA	-
LSI70_RS23785 (4618353)	4618353..4618850	-	498	Protein_4671	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4575713..4619799	44086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T227511 WP_001263495.1 NZ_CP089034:c4614265-4613867 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T227511 NZ_CP122929:819591-819998 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT227511 WP_000554757.1 NZ_CP089034:c4614561-4614268 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT227511 NZ_CP122929:819344-819610 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6