Detailed information of TA system TA022750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 460005..460642
• Replicon: chromosome
• Accession: NC_010513
• Organism: Xylella fastidiosa M12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: XFASM12_RS01940
• Protein ID: WP_004085174.1
• Coordinates: 460361..460642 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: XFASM12_RS01935
• Protein ID: WP_004087025.1
• Coordinates: 460005..460304 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFASM12_RS01910	456223..457815	-	1593	WP_012337641.1	tail fiber domain-containing protein	-
XFASM12_RS01915	457823..458380	-	558	WP_012337642.1	phage tail protein I	-
XFASM12_RS01920	458373..459266	-	894	WP_012337643.1	baseplate J/gp47 family protein	-
XFASM12_RS01925	459266..459604	-	339	WP_004088650.1	GPW/gp25 family protein	-
XFASM12_RS01930	459712..459993	+	282	WP_004087027.1	XRE family transcriptional regulator	-
XFASM12_RS01935	460005..460304	+	300	WP_004087025.1	HigA family addiction module antidote protein	Antitoxin
XFASM12_RS01940	460361..460642	+	282	WP_004085174.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XFASM12_RS01945	460653..460928	+	276	WP_012337645.1	HigA family addiction module antidote protein	-
XFASM12_RS01950	460933..461520	-	588	WP_012337646.1	phage baseplate assembly protein V	-
XFASM12_RS01955	461517..462059	-	543	WP_012337647.1	hypothetical protein	-
XFASM12_RS01960	462035..462556	-	522	WP_012337648.1	hypothetical protein	-
XFASM12_RS01965	462553..462876	-	324	WP_012337649.1	hypothetical protein	-
XFASM12_RS01970	462876..463136	-	261	WP_012337650.1	hypothetical protein	-
XFASM12_RS01975	463154..465028	-	1875	WP_012337651.1	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	446909..481283	34374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 9.8829

>T22750 WP_004085174.1 NC_010513:460361-460642 [Xylella fastidiosa M12]
MIKSFRHKGIQQFFLVGSTAGIQTKHAAKLRIQLTALESAKRPEDMNAPGWKLHPLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T22750 NC_010513:460361-460642 [Xylella fastidiosa M12]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTTGTGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCGCTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTACCACTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11022.77 Da
• Isoelectric Point: 9.0879

>AT22750 WP_004087025.1 NC_010513:460005-460304 [Xylella fastidiosa M12]
MTMSKTLPPIHPGEILREEFMVPLGLSSNALAKAIGVTAARINEIVRERRGITAETALRLARYFGTDPQSWLNLEQHYAL
ECAKRDLGDALSRIHPRVA

Nucleotide

• Length: 300 bp

>AT22750 NC_010513:460005-460304 [Xylella fastidiosa M12]
ATGACTATGTCTAAGACACTTCCCCCCATTCACCCAGGAGAAATTCTCCGGGAGGAGTTTATGGTCCCTCTAGGTCTGTC
CAGTAATGCGCTGGCTAAGGCCATTGGGGTGACGGCGGCACGCATCAATGAGATTGTGCGCGAGCGGCGCGGGATCACCG
CAGAGACTGCTTTGCGTCTGGCGCGCTACTTCGGTACCGATCCGCAAAGCTGGCTGAATTTGGAACAGCACTATGCGTTG
GAATGTGCCAAACGTGATCTTGGAGATGCGCTGTCACGGATTCATCCGCGAGTGGCTTGA