Detailed information of TA system TA022749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6837957..6838543
• Replicon: chromosome
• Accession: NC_010511
• Organism: Methylobacterium sp. 4-46

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B0UR34
• Locus tag: M446_RS30090
• Protein ID: WP_012335864.1
• Coordinates: 6838262..6838543 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M446_RS30085
• Protein ID: WP_018263550.1
• Coordinates: 6837957..6838247 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M446_RS30065	6833225..6833749	+	525	WP_012335859.1	histidine phosphatase family protein	-
M446_RS30070	6834018..6835373	+	1356	WP_012335860.1	hypothetical protein	-
M446_RS30075	6835494..6836939	+	1446	WP_012335861.1	YcjX family protein	-
M446_RS30080	6836936..6837946	+	1011	WP_012335862.1	TIGR01620 family protein	-
M446_RS30085	6837957..6838247	-	291	WP_018263550.1	HigA family addiction module antidote protein	Antitoxin
M446_RS30090	6838262..6838543	-	282	WP_012335864.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M446_RS30095	6838777..6839949	-	1173	WP_012335865.1	4-hydroxybenzoate 3-monooxygenase	-
M446_RS30100	6840112..6840999	+	888	WP_012335866.1	helix-turn-helix domain-containing protein	-
M446_RS30105	6841005..6841913	+	909	WP_012335867.1	aldo/keto reductase	-
M446_RS30110	6842173..6842577	+	405	WP_012335868.1	hypothetical protein	-
M446_RS30115	6842611..6843102	-	492	WP_012335869.1	tRNA (cytidine(34)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-80)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10783.15 Da
• Isoelectric Point: 6.3228

>T22749 WP_012335864.1 NC_010511:c6838543-6838262 [Methylobacterium sp. 4-46]
MIQSFADKHARALHEDGICHPRWRAFERIALRKLDALDAAVDLDDLRSPPGNRLEPLQGDRAGQHSIRINDQWRICFRWT
DLGPEEVAIVDYH

Nucleotide

• Length: 282 bp

>T22749 NC_010511:c6838543-6838262 [Methylobacterium sp. 4-46]
ATGATCCAGAGCTTCGCTGACAAGCACGCGCGCGCCCTGCATGAGGATGGCATCTGTCATCCGCGGTGGCGGGCATTCGA
ACGTATCGCCCTGCGCAAGCTCGATGCGCTCGACGCCGCGGTCGACCTCGACGACCTGCGCAGCCCGCCAGGCAATCGTT
TGGAACCTCTCCAGGGCGACCGCGCGGGCCAGCACAGCATTCGCATCAACGACCAATGGCGCATCTGCTTTCGCTGGACA
GACCTCGGTCCCGAGGAGGTCGCGATCGTCGATTATCATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10910.44 Da
• Isoelectric Point: 10.4025

>AT22749 WP_018263550.1 NC_010511:c6838247-6837957 [Methylobacterium sp. 4-46]
MPRIRTHPGEVLLEDFMKPFGLSARQLAEAIRVPHNRIGDILRERRSITADTALRLGRYFGTTAQFWVNLQTAHDLSKAA
ATHDYETIRPREAAPT

Nucleotide

• Length: 291 bp

>AT22749 NC_010511:c6838247-6837957 [Methylobacterium sp. 4-46]
ATGCCGCGCATCCGCACCCATCCTGGCGAAGTCCTCCTTGAGGACTTCATGAAACCGTTCGGCCTGAGCGCCCGCCAACT
CGCGGAGGCGATCAGGGTGCCGCACAACCGCATCGGCGACATCCTGCGCGAGCGCCGGAGCATCACGGCCGACACGGCCC
TCCGGCTCGGCCGCTACTTCGGCACCACGGCCCAGTTCTGGGTCAACCTCCAGACCGCGCACGATCTCTCGAAGGCGGCG
GCGACGCACGATTACGAGACGATCCGCCCGCGCGAGGCGGCTCCCACCTGA

Structures

Toxin

• AlphaFold DB: B0UR34