227478

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3404326..3405121 Replicon chromosome
Accession NZ_CP089032
Organism Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag LSI69_RS17635 Protein ID WP_000854914.1
Coordinates 3404326..3404700 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag LSI69_RS17640 Protein ID WP_001280954.1
Coordinates 3404747..3405121 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI69_RS17595 (3399330) 3399330..3399821 - 492 WP_001301587.1 DUF1097 domain-containing protein -
LSI69_RS17600 (3399923) 3399923..3400477 - 555 WP_001001917.1 molecular chaperone YcdY -
LSI69_RS17605 (3400501) 3400501..3401238 - 738 WP_000283664.1 zinc-binding phosphatase -
LSI69_RS17610 (3401293) 3401293..3402231 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
LSI69_RS17620 (3402702) 3402702..3403544 - 843 WP_001280481.1 DUF4942 domain-containing protein -
LSI69_RS17625 (3403629) 3403629..3403826 - 198 WP_000839282.1 DUF957 domain-containing protein -
LSI69_RS17630 (3403838) 3403838..3404329 - 492 WP_000976853.1 DUF5983 family protein -
LSI69_RS17635 (3404326) 3404326..3404700 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
LSI69_RS17640 (3404747) 3404747..3405121 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LSI69_RS17645 (3405284) 3405284..3405505 - 222 WP_001220314.1 DUF987 domain-containing protein -
LSI69_RS17650 (3405568) 3405568..3406044 - 477 WP_001186738.1 RadC family protein -
LSI69_RS17655 (3406060) 3406060..3406545 - 486 WP_000214398.1 antirestriction protein -
LSI69_RS17660 (3406636) 3406636..3407454 - 819 WP_001234682.1 DUF932 domain-containing protein -
LSI69_RS17665 (3407794) 3407794..3408864 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
LSI69_RS17670 (3408861) 3408861..3409766 - 906 WP_000203541.1 diguanylate cyclase regulator RdcB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3395712..3406545 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T227478 WP_000854914.1 NZ_CP089032:c3404700-3404326 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T227478 NZ_CP122881:10875-11027 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT227478 WP_001280954.1 NZ_CP089032:c3405121-3404747 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT227478 NZ_CP122881:c10825-10763 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References