Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 758193..758920 Replicon chromosome
Accession NZ_CP089032
Organism Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LSI69_RS03785 Protein ID WP_000550189.1
Coordinates 758193..758507 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LSI69_RS03790 Protein ID WP_000560249.1
Coordinates 758504..758920 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI69_RS03765 (754351) 754351..755337 - 987 WP_000617675.1 Gfo/Idh/MocA family oxidoreductase -
LSI69_RS03770 (755416) 755416..756108 - 693 WP_000942543.1 vancomycin high temperature exclusion protein -
LSI69_RS03775 (756185) 756185..756688 - 504 WP_001295542.1 M48 family metallopeptidase -
LSI69_RS03780 (756773) 756773..757909 + 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LSI69_RS03785 (758193) 758193..758507 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LSI69_RS03790 (758504) 758504..758920 + 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LSI69_RS03795 (758965) 758965..760983 - 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LSI69_RS03800 (761509) 761509..763860 - 2352 WP_000695517.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T227465 WP_000550189.1 NZ_CP089032:758193-758507 [Escherichia coli O157:H7]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T227465 NZ_CP122880:2046796-2047047 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCATAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT227465 WP_000560249.1 NZ_CP089032:758504-758920 [Escherichia coli O157:H7]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT227465 NZ_CP122880:2047044-2047298 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References