Detailed information of TA system TA227464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 715243..716042
• Replicon: chromosome
• Accession: NZ_CP089032
• Organism: Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: LSI69_RS03560
• Protein ID: WP_000347273.1
• Coordinates: 715243..715707 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: LSI69_RS03565
• Protein ID: WP_001302819.1
• Coordinates: 715707..716042 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI69_RS03530 (710244)	710244..710678	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
LSI69_RS03535 (710696)	710696..711574	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
LSI69_RS03540 (711564)	711564..712343	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
LSI69_RS03545 (712354)	712354..712827	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
LSI69_RS03550 (712850)	712850..714130	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
LSI69_RS03555 (714379)	714379..715188	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
LSI69_RS03560 (715243)	715243..715707	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
LSI69_RS03565 (715707)	715707..716042	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
LSI69_RS03570 (716191)	716191..717762	-	1572	WP_001273776.1	galactarate dehydratase	-
LSI69_RS03575 (718137)	718137..719471	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
LSI69_RS03580 (719487)	719487..720257	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T227464 WP_000347273.1 NZ_CP089032:c715707-715243 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T227464 NZ_CP122880:1093866-1094201 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT227464 WP_001302819.1 NZ_CP089032:c716042-715707 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT227464 NZ_CP122880:1093618-1093866 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: V0SSH7