Detailed information of TA system TA227455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4351499..4352193
• Replicon: chromosome
• Accession: NZ_CP089031
• Organism: Escherichia coli strain 7386Nal

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: CO543_RS22115
• Protein ID: WP_001263495.1
• Coordinates: 4351499..4351897 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CO543_RS22120
• Protein ID: WP_000554757.1
• Coordinates: 4351900..4352193 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4347086)	4347086..4347166	-	81	NuclAT_11	-	-
- (4347086)	4347086..4347166	-	81	NuclAT_11	-	-
- (4347086)	4347086..4347166	-	81	NuclAT_11	-	-
- (4347086)	4347086..4347166	-	81	NuclAT_11	-	-
CO543_RS22090 (4347762)	4347762..4348220	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CO543_RS22095 (4348481)	4348481..4349938	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CO543_RS22100 (4349995)	4349995..4350516	-	522	Protein_4342	peptide chain release factor H	-
CO543_RS22105 (4350515)	4350515..4350718	-	204	Protein_4343	RtcB family protein	-
CO543_RS22110 (4351037)	4351037..4351489	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
CO543_RS22115 (4351499)	4351499..4351897	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CO543_RS22120 (4351900)	4351900..4352193	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CO543_RS22125 (4352245)	4352245..4353300	-	1056	WP_001226186.1	DNA polymerase IV	-
CO543_RS22130 (4353371)	4353371..4354156	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CO543_RS22135 (4354128)	4354128..4355840	+	1713	Protein_4349	flagellar biosynthesis protein FlhA	-
CO543_RS22140 (4355985)	4355985..4356482	-	498	Protein_4350	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T227455 WP_001263495.1 NZ_CP089031:c4351897-4351499 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T227455 NZ_CP122878:78318-78716 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT227455 WP_000554757.1 NZ_CP089031:c4352193-4351900 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT227455 NZ_CP122878:78091-78318 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6