Detailed information of TA system TA227450

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3464861..3465086
• Replicon: chromosome
• Accession: NZ_CP089031
• Organism: Escherichia coli strain 7386Nal

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: CO543_RS17965
• Protein ID: WP_000813263.1
• Coordinates: 3464861..3465016 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3465028..3465086 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO543_RS17930	3460315..3461028	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
CO543_RS17935	3461166..3461362	-	197	Protein_3525	TrmB family transcriptional regulator	-
CO543_RS17940	3461649..3462467	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
CO543_RS17945	3462619..3462990	-	372	WP_000090264.1	antiterminator Q family protein	-
CO543_RS17950	3462980..3463351	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
CO543_RS17955	3463364..3464413	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
CO543_RS17960	3464415..3464693	-	279	WP_001341388.1	hypothetical protein	-
CO543_RS17965	3464861..3465016	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3465028..3465086	+	59	-	-	Antitoxin
CO543_RS17975	3465621..3466394	+	774	WP_000160654.1	alpha/beta hydrolase	-
CO543_RS17980	3466746..3467159	-	414	WP_001151233.1	DUF977 family protein	-
CO543_RS17985	3467175..3467945	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
CO543_RS17990	3467967..3468713	-	747	WP_000788745.1	ATP-binding protein	-
CO543_RS17995	3468720..3469817	-	1098	WP_001475117.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T227450 WP_000813263.1 NZ_CP089031:c3465016-3464861 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T227450 NZ_CP122876:4063902-4064180 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT227450 NZ_CP089031:3465028-3465086 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin