Detailed information of TA system TA227438

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2642568..2642793
• Replicon: chromosome
• Accession: NZ_CP089031
• Organism: Escherichia coli strain 7386Nal

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: CO543_RS13460
• Protein ID: WP_000813258.1
• Coordinates: 2642568..2642723 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2642735..2642793 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO543_RS13410	2637571..2638002	-	432	WP_001302123.1	tellurite resistance protein	-
CO543_RS13425	2638453..2639166	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
CO543_RS13430	2639302..2639499	-	198	WP_000917763.1	hypothetical protein	-
CO543_RS13435	2639724..2640278	-	555	WP_000640035.1	DUF1133 family protein	-
CO543_RS13440	2640341..2640646	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
CO543_RS13445	2640659..2641708	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
CO543_RS13450	2641710..2641982	-	273	WP_000191872.1	hypothetical protein	-
CO543_RS13455	2642104..2642448	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
CO543_RS13460	2642568..2642723	-	156	WP_000813258.1	Hok/Gef family protein	Toxin
-	2642735..2642793	+	59	-	-	Antitoxin
CO543_RS13465	2643014..2643571	-	558	WP_000104474.1	DUF551 domain-containing protein	-
CO543_RS13470	2643573..2643791	-	219	WP_000683609.1	DUF4014 family protein	-
CO543_RS13475	2643919..2644230	-	312	WP_001289673.1	hypothetical protein	-
CO543_RS13480	2644223..2644450	-	228	WP_000699809.1	hypothetical protein	-
CO543_RS13485	2644447..2644728	-	282	WP_000603384.1	DNA-binding protein	-
CO543_RS13490	2644761..2645477	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
CO543_RS13495	2645511..2645972	-	462	WP_000139447.1	replication protein P	-
CO543_RS13500	2645965..2647008	-	1044	WP_001262402.1	DnaT-like ssDNA-binding domain-containing protein	-
CO543_RS13505	2647077..2647502	-	426	WP_000693878.1	toxin YdaT family protein	-
CO543_RS13510	2647486..2647728	-	243	WP_000747948.1	Cro/CI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2612114..2699614	87500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T227438 WP_000813258.1 NZ_CP089031:c2642723-2642568 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T227438 NZ_CP122874:90881-91030 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT227438 NZ_CP089031:2642735-2642793 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin