Detailed information of TA system TA227381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13609..14266
• Replicon: plasmid p53374-177.1
• Accession: NZ_CP089019
• Organism: Klebsiella pneumoniae strain 53374

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: LRZ80_RS28410
• Protein ID: WP_000270043.1
• Coordinates: 13916..14266 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRZ80_RS28405
• Protein ID: WP_000124640.1
• Coordinates: 13609..13911 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ80_RS28360 (LRZ80_28365)	9234..9662	+	429	WP_000591074.1	hypothetical protein	-
LRZ80_RS28365 (LRZ80_28370)	9719..10078	+	360	WP_000422768.1	hypothetical protein	-
LRZ80_RS28370 (LRZ80_28375)	10078..10524	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
LRZ80_RS28375 (LRZ80_28380)	10521..11039	+	519	WP_000210756.1	nitrite reductase	-
LRZ80_RS28380 (LRZ80_28385)	11039..11269	+	231	WP_000972663.1	hypothetical protein	-
LRZ80_RS28385 (LRZ80_28390)	11256..12113	+	858	WP_001167032.1	hypothetical protein	-
LRZ80_RS28390 (LRZ80_28395)	12344..12871	+	528	WP_001236377.1	thermonuclease family protein	-
LRZ80_RS28395 (LRZ80_28400)	12929..13201	+	273	WP_001043047.1	HU family DNA-binding protein	-
LRZ80_RS28400 (LRZ80_28405)	13289..13582	+	294	WP_001239997.1	chromosome segregation protein ParM	-
LRZ80_RS28405 (LRZ80_28410)	13609..13911	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
LRZ80_RS28410 (LRZ80_28415)	13916..14266	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRZ80_RS28415 (LRZ80_28420)	14429..14977	+	549	WP_001061574.1	transcriptional regulator	-
LRZ80_RS28420 (LRZ80_28425)	15318..15512	+	195	WP_000343597.1	hypothetical protein	-
LRZ80_RS28425 (LRZ80_28430)	15523..15894	+	372	WP_000516916.1	hypothetical protein	-
LRZ80_RS28430 (LRZ80_28435)	15887..16357	+	471	WP_001281821.1	hypothetical protein	-
LRZ80_RS28435 (LRZ80_28440)	16372..16707	-	336	WP_000683476.1	hypothetical protein	-
LRZ80_RS28440 (LRZ80_28445)	16804..17292	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
LRZ80_RS28445 (LRZ80_28450)	17295..17792	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / floR / tet(G) / rmtB / blaTEM-1B / erm(42) / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14	-	1..177113	177113

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T227381 WP_000270043.1 NZ_CP089019:c14266-13916 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T227381 NZ_CP122859:c2488029-2487853 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT227381 WP_000124640.1 NZ_CP089019:c13911-13609 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT227381 NZ_CP122859:c2487807-2487391 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9