Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 351316..351962 Replicon chromosome
Accession NZ_CP089014
Organism Klebsiella pneumoniae strain 53374

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag LRZ80_RS01625 Protein ID WP_002920560.1
Coordinates 351316..351663 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag LRZ80_RS01630 Protein ID WP_002920557.1
Coordinates 351663..351962 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ80_RS01615 (LRZ80_01615) 347242..348675 + 1434 WP_002920564.1 glycogen synthase GlgA -
LRZ80_RS01620 (LRZ80_01620) 348693..351140 + 2448 WP_002920561.1 glycogen phosphorylase -
LRZ80_RS01625 (LRZ80_01625) 351316..351663 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LRZ80_RS01630 (LRZ80_01630) 351663..351962 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
LRZ80_RS01635 (LRZ80_01635) 352025..353533 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
LRZ80_RS01640 (LRZ80_01640) 353738..354067 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LRZ80_RS01645 (LRZ80_01645) 354118..354948 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
LRZ80_RS01650 (LRZ80_01650) 354998..355756 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T227363 WP_002920560.1 NZ_CP089014:351316-351663 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T227363 NZ_CP122847:57327-57602 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT227363 WP_002920557.1 NZ_CP089014:351663-351962 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT227363 NZ_CP122847:57043-57327 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References