Detailed information of TA system TA022736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3506838..3507637
• Replicon: chromosome
• Accession: NC_010498
• Organism: Escherichia coli SMS-3-5

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: ECSMS35_RS17300
• Protein ID: WP_000347273.1
• Coordinates: 3507173..3507637 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: ECSMS35_RS17295
• Protein ID: WP_021547164.1
• Coordinates: 3506838..3507173 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSMS35_RS17275	3502623..3503393	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
ECSMS35_RS17280	3503409..3504743	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
ECSMS35_RS17290	3505118..3506689	+	1572	WP_001273756.1	galactarate dehydratase	-
ECSMS35_RS17295	3506838..3507173	+	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ECSMS35_RS17300	3507173..3507637	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ECSMS35_RS17305	3507692..3508501	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
ECSMS35_RS17310	3508750..3510030	+	1281	WP_000681958.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ECSMS35_RS17315	3510053..3510526	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ECSMS35_RS17320	3510537..3511316	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ECSMS35_RS17325	3511306..3512184	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ECSMS35_RS17330	3512202..3512636	+	435	WP_000948834.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3496355..3507637	11282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T22736 WP_000347273.1 NC_010498:3507173-3507637 [Escherichia coli SMS-3-5]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T22736 NC_010498:3507173-3507637 [Escherichia coli SMS-3-5]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT22736 WP_021547164.1 NC_010498:3506838-3507173 [Escherichia coli SMS-3-5]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT22736 NC_010498:3506838-3507173 [Escherichia coli SMS-3-5]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39