Detailed information of TA system TA227335

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26159..26428
• Replicon: plasmid p56661-89.7
• Accession: NZ_CP089010
• Organism: Klebsiella pneumoniae strain 56661

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LRZ77_RS27840
• Protein ID: WP_001372321.1
• Coordinates: 26303..26428 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26159..26224 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ77_RS27810	21911..22396	+	486	WP_031311812.1	single-stranded DNA-binding protein	-
LRZ77_RS27815	22454..22687	+	234	WP_000006003.1	DUF905 family protein	-
LRZ77_RS27820	22748..24771	+	2024	Protein_27	ParB/RepB/Spo0J family partition protein	-
LRZ77_RS27825	24840..25274	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LRZ77_RS27830	25271..25990	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	26002..26226	+	225	NuclAT_0	-	-
-	26002..26226	+	225	NuclAT_0	-	-
-	26002..26226	+	225	NuclAT_0	-	-
-	26002..26226	+	225	NuclAT_0	-	-
-	26159..26224	-	66	-	-	Antitoxin
LRZ77_RS27835	26212..26361	+	150	Protein_30	plasmid maintenance protein Mok	-
LRZ77_RS27840	26303..26428	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LRZ77_RS27845	26786..27211	+	426	WP_000422741.1	transposase	-
LRZ77_RS27850	27208..27558	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
LRZ77_RS27855	27589..29202	+	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
LRZ77_RS27860	29287..29583	-	297	Protein_35	hypothetical protein	-
LRZ77_RS27865	29883..30179	+	297	WP_001272251.1	hypothetical protein	-
LRZ77_RS27870	30290..31111	+	822	WP_001234445.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T227335 WP_001372321.1 NZ_CP089010:26303-26428 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T227335 NZ_CP122837:c4762552-4762241 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT227335 NZ_CP089010:c26224-26159 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin