Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 332341..332927 Replicon chromosome
Accession NZ_CP089001
Organism Klebsiella pneumoniae strain 56661

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag LRZ77_RS01535 Protein ID WP_002920800.1
Coordinates 332559..332927 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LRZ77_RS01530 Protein ID WP_004174006.1
Coordinates 332341..332562 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ77_RS01510 (328498) 328498..329424 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LRZ77_RS01515 (329421) 329421..330698 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
LRZ77_RS01520 (330695) 330695..331462 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LRZ77_RS01525 (331464) 331464..332177 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LRZ77_RS01530 (332341) 332341..332562 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LRZ77_RS01535 (332559) 332559..332927 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LRZ77_RS01540 (333200) 333200..334516 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LRZ77_RS01545 (334623) 334623..335510 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LRZ77_RS01550 (335507) 335507..336352 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LRZ77_RS01555 (336354) 336354..337424 + 1071 WP_032433652.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 329421..338161 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T227313 WP_002920800.1 NZ_CP089001:332559-332927 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T227313 NZ_CP122834:23075-23224 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT227313 WP_004174006.1 NZ_CP089001:332341-332562 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT227313 NZ_CP122834:c23031-22970 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References