Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 60065..60792 | Replicon | plasmid p50700-217.1 |
| Accession | NZ_CP088996 | ||
| Organism | Klebsiella pneumoniae strain 50700 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | LRZ81_RS28835 | Protein ID | WP_011251285.1 |
| Coordinates | 60481..60792 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LRZ81_RS28830 | Protein ID | WP_011251286.1 |
| Coordinates | 60065..60484 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LRZ81_RS28800 (LRZ81_28800) | 55233..55703 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| LRZ81_RS28805 (LRZ81_28805) | 56016..56651 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| LRZ81_RS28810 (LRZ81_28810) | 57070..57690 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| LRZ81_RS28815 (LRZ81_28815) | 57711..58499 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| LRZ81_RS28820 (LRZ81_28820) | 58513..58878 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| LRZ81_RS28825 (LRZ81_28825) | 58950..59918 | - | 969 | WP_074428168.1 | IS5 family transposase | - |
| LRZ81_RS28830 (LRZ81_28830) | 60065..60484 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| LRZ81_RS28835 (LRZ81_28835) | 60481..60792 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| LRZ81_RS28840 (LRZ81_28840) | 60997..61434 | - | 438 | Protein_61 | DDE-type integrase/transposase/recombinase | - |
| LRZ81_RS28845 (LRZ81_28845) | 61569..62266 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| LRZ81_RS28850 (LRZ81_28850) | 62268..62717 | + | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| LRZ81_RS28855 (LRZ81_28855) | 62734..63045 | + | 312 | WP_011251282.1 | hypothetical protein | - |
| LRZ81_RS28860 (LRZ81_28860) | 63059..63400 | + | 342 | WP_011251281.1 | hypothetical protein | - |
| LRZ81_RS28865 (LRZ81_28865) | 63460..64416 | + | 957 | WP_011251280.1 | DsbA family protein | - |
| LRZ81_RS28870 (LRZ81_28870) | 64841..65281 | - | 441 | WP_011251275.1 | hypothetical protein | - |
| LRZ81_RS28875 (LRZ81_28875) | 65287..65781 | - | 495 | WP_011251274.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..217070 | 217070 | |
| - | inside | IScluster/Tn | - | - | 43395..67701 | 24306 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T227284 WP_011251285.1 NZ_CP088996:c60792-60481 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T227284 NZ_CP122823:3948535-3948753 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT227284 WP_011251286.1 NZ_CP088996:c60484-60065 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT227284 NZ_CP122823:3948135-3948509 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|