Detailed information of TA system TA227279

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29094..29363
• Replicon: plasmid p50700-140.6
• Accession: NZ_CP088995
• Organism: Klebsiella pneumoniae strain 50700

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LRZ81_RS27805
• Protein ID: WP_001372321.1
• Coordinates: 29238..29363 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	29094..29159 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ81_RS27770	24096..24557	-	462	Protein_31	hypothetical protein	-
LRZ81_RS27775	24859..25386	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LRZ81_RS27780	25444..25677	+	234	WP_000006003.1	DUF905 family protein	-
LRZ81_RS27785	25738..27706	+	1969	Protein_34	ParB/RepB/Spo0J family partition protein	-
LRZ81_RS27790	27775..28209	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LRZ81_RS27795	28206..28925	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	28937..29161	+	225	NuclAT_0	-	-
-	28937..29161	+	225	NuclAT_0	-	-
-	28937..29161	+	225	NuclAT_0	-	-
-	28937..29161	+	225	NuclAT_0	-	-
-	29094..29159	-	66	-	-	Antitoxin
LRZ81_RS27800	29147..29296	+	150	Protein_37	plasmid maintenance protein Mok	-
LRZ81_RS27805	29238..29363	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LRZ81_RS27810	29682..29978	-	297	Protein_39	hypothetical protein	-
LRZ81_RS27815	30278..30574	+	297	WP_001272251.1	hypothetical protein	-
LRZ81_RS27820	30685..31506	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LRZ81_RS27825	31803..32450	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LRZ81_RS27830	32727..33110	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LRZ81_RS27835	33301..33987	+	687	WP_015059009.1	PAS domain-containing protein	-
LRZ81_RS27840	34081..34308	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaSHV-12 / blaKPC-2	-	1..140550	140550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T227279 WP_001372321.1 NZ_CP088995:29238-29363 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T227279 NZ_CP122823:2277697-2277800 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 66 bp

>AT227279 NZ_CP088995:c29159-29094 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin