Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 332346..332932 Replicon chromosome
Accession NZ_CP088988
Organism Klebsiella pneumoniae strain 53322

Toxin (Protein)


Gene name doc Uniprot ID A0A7Z9B6M3
Locus tag LRZ75_RS01540 Protein ID WP_029884514.1
Coordinates 332564..332932 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LRZ75_RS01535 Protein ID WP_004174006.1
Coordinates 332346..332567 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ75_RS01515 (328503) 328503..329429 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LRZ75_RS01520 (329426) 329426..330703 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
LRZ75_RS01525 (330700) 330700..331467 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LRZ75_RS01530 (331469) 331469..332182 + 714 WP_029884513.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LRZ75_RS01535 (332346) 332346..332567 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LRZ75_RS01540 (332564) 332564..332932 + 369 WP_029884514.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LRZ75_RS01545 (333205) 333205..334521 + 1317 WP_029884515.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LRZ75_RS01550 (334628) 334628..335515 + 888 WP_004901104.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LRZ75_RS01555 (335512) 335512..336357 + 846 WP_004901101.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LRZ75_RS01560 (336359) 336359..337429 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 330700..338166 7466


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13583.01 Da        Isoelectric Point: 8.6410

>T227240 WP_029884514.1 NZ_CP088988:332564-332932 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRMALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T227240 NZ_CP122807:96889-97170 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAGATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT227240 WP_004174006.1 NZ_CP088988:332346-332567 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT227240 NZ_CP122807:96648-96899 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z9B6M3

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References