Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3649083..3649680 Replicon chromosome
Accession NZ_CP088985
Organism Klebsiella pneumoniae strain 53334

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LRZ83_RS17755 Protein ID WP_004142563.1
Coordinates 3649363..3649680 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LRZ83_RS17750 Protein ID WP_004142561.1
Coordinates 3649083..3649370 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ83_RS17720 (LRZ83_17720) 3645163..3645411 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LRZ83_RS17725 (LRZ83_17725) 3645429..3645770 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LRZ83_RS17730 (LRZ83_17730) 3645801..3646916 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
LRZ83_RS17735 (LRZ83_17735) 3647096..3647677 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LRZ83_RS17740 (LRZ83_17740) 3647677..3648045 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LRZ83_RS17745 (LRZ83_17745) 3648165..3648818 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LRZ83_RS17750 (LRZ83_17750) 3649083..3649370 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LRZ83_RS17755 (LRZ83_17755) 3649363..3649680 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LRZ83_RS17760 (LRZ83_17760) 3649865..3650908 - 1044 WP_020802450.1 DUF2157 domain-containing protein -
LRZ83_RS17765 (LRZ83_17765) 3651578..3652444 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LRZ83_RS17770 (LRZ83_17770) 3652553..3653980 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T227229 WP_004142563.1 NZ_CP088985:c3649680-3649363 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T227229 NZ_CP122801:3948520-3948738 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT227229 WP_004142561.1 NZ_CP088985:c3649370-3649083 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT227229 NZ_CP122801:3948120-3948494 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References