Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 363152..363798 Replicon chromosome
Accession NZ_CP088985
Organism Klebsiella pneumoniae strain 53334

Toxin (Protein)


Gene name higB Uniprot ID A0A486RBU5
Locus tag LRZ83_RS01685 Protein ID WP_032410616.1
Coordinates 363152..363499 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag LRZ83_RS01690 Protein ID WP_002920557.1
Coordinates 363499..363798 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ83_RS01675 (LRZ83_01675) 359078..360511 + 1434 WP_002920564.1 glycogen synthase GlgA -
LRZ83_RS01680 (LRZ83_01680) 360529..362976 + 2448 WP_002920561.1 glycogen phosphorylase -
LRZ83_RS01685 (LRZ83_01685) 363152..363499 + 348 WP_032410616.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LRZ83_RS01690 (LRZ83_01690) 363499..363798 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
LRZ83_RS01695 (LRZ83_01695) 363861..365369 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
LRZ83_RS01700 (LRZ83_01700) 365574..365903 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LRZ83_RS01705 (LRZ83_01705) 365954..366784 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
LRZ83_RS01710 (LRZ83_01710) 366834..367592 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13545.55 Da        Isoelectric Point: 5.6802

>T227218 WP_032410616.1 NZ_CP088985:363152-363499 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATILILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFEPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T227218 NZ_CP122801:c643360-642896 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT227218 WP_002920557.1 NZ_CP088985:363499-363798 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT227218 NZ_CP122801:c643695-643360 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486RBU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References