Detailed information of TA system TA227214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 57413..58140
• Replicon: plasmid p53357-227.9
• Accession: NZ_CP088984
• Organism: Klebsiella pneumoniae strain 53357

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LRZ78_RS27180
• Protein ID: WP_011251285.1
• Coordinates: 57829..58140 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRZ78_RS27175
• Protein ID: WP_011251286.1
• Coordinates: 57413..57832 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ78_RS27145 (LRZ78_27150)	52581..53051	-	471	WP_048333570.1	hypothetical protein	-
LRZ78_RS27150 (LRZ78_27155)	53364..53999	-	636	WP_223171879.1	hypothetical protein	-
LRZ78_RS27155 (LRZ78_27160)	54418..55038	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LRZ78_RS27160 (LRZ78_27165)	55059..55847	+	789	WP_040217257.1	hypothetical protein	-
LRZ78_RS27165 (LRZ78_27170)	55861..56226	+	366	WP_048333448.1	hypothetical protein	-
LRZ78_RS27170 (LRZ78_27175)	56298..57266	-	969	WP_086642568.1	IS5 family transposase	-
LRZ78_RS27175 (LRZ78_27180)	57413..57832	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LRZ78_RS27180 (LRZ78_27185)	57829..58140	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LRZ78_RS27185 (LRZ78_27190)	58345..58782	-	438	Protein_58	DDE-type integrase/transposase/recombinase	-
LRZ78_RS27190 (LRZ78_27195)	58917..59614	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LRZ78_RS27195 (LRZ78_27200)	59616..60065	+	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
LRZ78_RS27200 (LRZ78_27205)	60082..60393	+	312	WP_011251282.1	hypothetical protein	-
LRZ78_RS27205 (LRZ78_27210)	60407..60748	+	342	WP_011251281.1	hypothetical protein	-
LRZ78_RS27210 (LRZ78_27215)	60808..61764	+	957	WP_011251280.1	DsbA family protein	-
LRZ78_RS27215 (LRZ78_27220)	61881..62456	+	576	WP_074424927.1	hypothetical protein	-
LRZ78_RS27220 (LRZ78_27225)	62564..63079	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..227891	227891
	-	inside	IScluster/Tn	-	-	47292..59614	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T227214 WP_011251285.1 NZ_CP088984:c58140-57829 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T227214 NZ_CP122770:c93156-93004 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT227214 WP_011251286.1 NZ_CP088984:c57832-57413 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT227214 NZ_CP122770:93206-93268 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1