Detailed information of TA system TA227195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3511418..3512008
• Replicon: chromosome
• Accession: NZ_CP088980
• Organism: Rhodanobacter denitrificans strain FW104-R5

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: LRK52_RS16245
• Protein ID: WP_027490439.1
• Coordinates: 3511727..3512008 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK52_RS16240
• Protein ID: WP_007512782.1
• Coordinates: 3511418..3511717 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK52_RS16225 (LRK52_16225)	3507230..3507814	+	585	WP_027490441.1	hypothetical protein	-
LRK52_RS16230 (LRK52_16230)	3507901..3508065	-	165	Protein_3195	glycerophosphodiester phosphodiesterase family protein	-
LRK52_RS16235 (LRK52_16235)	3508128..3511364	-	3237	WP_027490440.1	type I restriction endonuclease subunit R	-
LRK52_RS16240 (LRK52_16240)	3511418..3511717	-	300	WP_007512782.1	HigA family addiction module antitoxin	Antitoxin
LRK52_RS16245 (LRK52_16245)	3511727..3512008	-	282	WP_027490439.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK52_RS16250 (LRK52_16250)	3512088..3513368	-	1281	WP_027490438.1	restriction endonuclease subunit S	-
LRK52_RS16255 (LRK52_16255)	3513365..3515440	-	2076	WP_027490437.1	class I SAM-dependent DNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10745.42 Da
• Isoelectric Point: 9.3345

>T227195 WP_027490439.1 NZ_CP088980:c3512008-3511727 [Rhodanobacter denitrificans]
MTIQSFCCKDTQALFERRRVRRFLVIEAAARRKLELLNAATQLDYLRVPPGNRLEALAGDRKGQHSIRVNDQFRLCFVWT
ADGPGLVEIVDYH

Nucleotide

• Length: 282 bp

>T227195 NZ_CP122768:c887987-887610 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.79 Da
• Isoelectric Point: 7.9969

>AT227195 WP_007512782.1 NZ_CP088980:c3511717-3511418 [Rhodanobacter denitrificans]
MPRKLPPIHPGEILREEYMLPLELSSNALARALEVTPARINEIVRERRGISADTALRLARYFGNDAQSWLNLQQRYDIEC
AQDSIGGALRKITPYQAAA

Nucleotide

• Length: 300 bp

>AT227195 NZ_CP122768:c888445-888077 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCACCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA