Detailed information of TA system TA227194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1389248..1389855
• Replicon: chromosome
• Accession: NZ_CP088980
• Organism: Rhodanobacter denitrificans strain FW104-R5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4WTS9
• Locus tag: LRK52_RS06335
• Protein ID: WP_007510671.1
• Coordinates: 1389248..1389562 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK52_RS06340
• Protein ID: WP_015447258.1
• Coordinates: 1389577..1389855 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK52_RS06330 (LRK52_06330)	1385045..1388776	+	3732	WP_027489851.1	diguanylate cyclase	-
LRK52_RS06335 (LRK52_06335)	1389248..1389562	+	315	WP_007510671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK52_RS06340 (LRK52_06340)	1389577..1389855	+	279	WP_015447258.1	helix-turn-helix domain-containing protein	Antitoxin
LRK52_RS06345 (LRK52_06345)	1389911..1390342	-	432	WP_027489850.1	Arc family DNA-binding protein	-
LRK52_RS06350 (LRK52_06350)	1390431..1390793	+	363	WP_015447260.1	hypothetical protein	-
LRK52_RS06355 (LRK52_06355)	1390858..1391622	+	765	WP_015447261.1	hypothetical protein	-
LRK52_RS06365 (LRK52_06365)	1391917..1392258	+	342	WP_007512800.1	lipid-A-disaccharide synthase N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11641.42 Da
• Isoelectric Point: 9.8349

>T227194 WP_007510671.1 NZ_CP088980:1389248-1389562 [Rhodanobacter denitrificans]
MIFVETPIFTAQIKDLLSDDDYGAFQRYLAENPEAGDVIKETGGLRKIRVAAKGHGKSGGARVIYYYFVSASRIAMVFAY
PKNVQENLTPAQKAALKKVIEQWR

Nucleotide

• Length: 315 bp

>T227194 NZ_CP122768:c690875-690411 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10263.75 Da
• Isoelectric Point: 7.2060

>AT227194 WP_015447258.1 NZ_CP088980:1389577-1389855 [Rhodanobacter denitrificans]
MFDNLMESVTQMDEIVRGERQPSREVHIDALAVKEIRRATGLSQAKFAGIVDVQVGTLRNWEQGRREPTGPAKALLRAIS
KDPKHVLEALAD

Nucleotide

• Length: 279 bp

>AT227194 NZ_CP122768:c691210-690875 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A154QK28