Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324027..324613 Replicon chromosome
Accession NZ_CP088978
Organism Klebsiella pneumoniae strain KP015

Toxin (Protein)


Gene name doc Uniprot ID A0A9J6Y968
Locus tag LST75_RS01515 Protein ID WP_025367836.1
Coordinates 324245..324613 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LST75_RS01510 Protein ID WP_004174006.1
Coordinates 324027..324248 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LST75_RS01490 (LST75_01490) 320184..321110 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LST75_RS01495 (LST75_01495) 321107..322384 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
LST75_RS01500 (LST75_01500) 322381..323148 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LST75_RS01505 (LST75_01505) 323150..323863 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LST75_RS01510 (LST75_01510) 324027..324248 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LST75_RS01515 (LST75_01515) 324245..324613 + 369 WP_025367836.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LST75_RS01520 (LST75_01520) 324886..326202 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LST75_RS01525 (LST75_01525) 326309..327196 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LST75_RS01530 (LST75_01530) 327193..328038 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LST75_RS01535 (LST75_01535) 328040..329110 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 321107..329847 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13449.86 Da        Isoelectric Point: 7.2790

>T227176 WP_025367836.1 NZ_CP088978:324245-324613 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIECEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLLG

Download         Length: 369 bp

>T227176 NZ_CP122758:2695457-2695564 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT227176 WP_004174006.1 NZ_CP088978:324027-324248 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT227176 NZ_CP122758:c2695410-2695344 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References