Detailed information of TA system TA227159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3399192..3399798
• Replicon: chromosome
• Accession: NZ_CP088935
• Organism: Thauera sp. WB-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3F3GK39
• Locus tag: LQF09_RS15605
• Protein ID: WP_002933838.1
• Coordinates: 3399475..3399798 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LQF09_RS15600
• Protein ID: WP_002945515.1
• Coordinates: 3399192..3399482 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQF09_RS15590 (LQF09_15595)	3394676..3396922	-	2247	WP_270068376.1	polysaccharide biosynthesis tyrosine autokinase	-
LQF09_RS15595 (LQF09_15600)	3397023..3398255	-	1233	WP_270068377.1	polysaccharide export protein	-
LQF09_RS15600 (LQF09_15605)	3399192..3399482	-	291	WP_002945515.1	helix-turn-helix domain-containing protein	Antitoxin
LQF09_RS15605 (LQF09_15610)	3399475..3399798	-	324	WP_002933838.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LQF09_RS15610 (LQF09_15615)	3399888..3400343	-	456	WP_270068378.1	transposase	-
LQF09_RS15615 (LQF09_15620)	3400557..3401933	-	1377	WP_270068379.1	lysine 2,3-aminomutase	-
LQF09_RS15620 (LQF09_15625)	3402336..3404324	-	1989	WP_270068380.1	acetyl-CoA carboxylase biotin carboxylase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	tviB	3372111..3416155	44044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (9-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-86)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12127.03 Da
• Isoelectric Point: 9.8121

>T227159 WP_002933838.1 NZ_CP088935:c3399798-3399475 [Thauera sp. WB-2]
MVFVELPIFSRCAQALFSDAQLRDLQQVLLENPSVGDLIPGGRGLRKFRVALLGRGKRGGARVIYYHWVSREQCYLIYAY
AKNARADLTKDQLRRLAEVIETEVSNG

Nucleotide

• Length: 324 bp

>T227159 NZ_CP122727:c4352799-4352626 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10877.60 Da
• Isoelectric Point: 10.6447

>AT227159 WP_002945515.1 NZ_CP088935:c3399482-3399192 [Thauera sp. WB-2]
MDDKHFEELLESVRDMGRHMRGEPVAGVVARELPEPDVKAIRERTGLSQTRFAYLIGVKPKTLQNWEQRRVRPAGPARAL
LKIVEANPAALSVLHR

Nucleotide

• Length: 291 bp

>AT227159 NZ_CP122727:c4353123-4352827 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A3F3GK39