Detailed information of TA system TA227150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1085715..1086314
• Replicon: chromosome
• Accession: NZ_CP088925
• Organism: Rhodanobacter denitrificans strain FW104-MT042

Toxin (Protein)

• Gene name: graT
• Uniprot ID: I4WT83
• Locus tag: LRK55_RS05055
• Protein ID: WP_007510748.1
• Coordinates: 1086033..1086314 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK55_RS05050
• Protein ID: WP_235644881.1
• Coordinates: 1085715..1086020 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK55_RS05030 (LRK55_05030)	1081158..1081388	-	231	WP_235644878.1	glutaredoxin family protein	-
LRK55_RS05035 (LRK55_05035)	1081464..1082846	+	1383	WP_235644879.1	L-serine ammonia-lyase	-
LRK55_RS05040 (LRK55_05040)	1082957..1083403	-	447	WP_027490858.1	hypothetical protein	-
LRK55_RS05045 (LRK55_05045)	1083496..1085577	+	2082	WP_235644880.1	site-specific DNA-methyltransferase	-
LRK55_RS05050 (LRK55_05050)	1085715..1086020	-	306	WP_235644881.1	HigA family addiction module antitoxin	Antitoxin
LRK55_RS05055 (LRK55_05055)	1086033..1086314	-	282	WP_007510748.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK55_RS05060 (LRK55_05060)	1086400..1089339	+	2940	WP_235644882.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (9-98)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10873.31 Da
• Isoelectric Point: 7.8618

>T227150 WP_007510748.1 NZ_CP088925:c1086314-1086033 [Rhodanobacter denitrificans]
VIRSFADKETARLSATGTSRRFPPDIVRWALSRLKYLHLATCPEDLREPFSNRLEMLSGNRAGQWSIRINDQWRLCFRFE
GGDAFDVEIVDYH

Nucleotide

• Length: 282 bp

>T227150 NZ_CP122727:1840025-1840276 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11225.98 Da
• Isoelectric Point: 7.4043

>AT227150 WP_235644881.1 NZ_CP088925:c1086020-1085715 [Rhodanobacter denitrificans]
MSIHATTNGLSPIHPGELLEEILSDHGMSQAAFARAIGVSPMRISHVIREERPVTAELALRFGRAFGQSPQFWMNMQTDY
DLKIAERELKASLRKVLPLAA

Nucleotide

• Length: 306 bp

>AT227150 NZ_CP122727:1840273-1840527 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATATCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: I4WT83