Detailed information of TA system TA227142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2315473..2316063
• Replicon: chromosome
• Accession: NZ_CP088922
• Organism: Rhodanobacter denitrificans strain FW104-10B01

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: LRK54_RS10485
• Protein ID: WP_027490439.1
• Coordinates: 2315473..2315754 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK54_RS10490
• Protein ID: WP_007512782.1
• Coordinates: 2315764..2316063 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK54_RS10475 (LRK54_10475)	2312041..2314116	+	2076	WP_027490437.1	class I SAM-dependent DNA methyltransferase	-
LRK54_RS10480 (LRK54_10480)	2314113..2315393	+	1281	WP_027490438.1	restriction endonuclease subunit S	-
LRK54_RS10485 (LRK54_10485)	2315473..2315754	+	282	WP_027490439.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK54_RS10490 (LRK54_10490)	2315764..2316063	+	300	WP_007512782.1	HigA family addiction module antitoxin	Antitoxin
LRK54_RS10495 (LRK54_10495)	2316117..2319353	+	3237	WP_027490440.1	type I restriction endonuclease subunit R	-
LRK54_RS10500 (LRK54_10500)	2319416..2319580	+	165	Protein_2068	glycerophosphodiester phosphodiesterase family protein	-
LRK54_RS10505 (LRK54_10505)	2319667..2320251	-	585	WP_027490441.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10745.42 Da
• Isoelectric Point: 9.3345

>T227142 WP_027490439.1 NZ_CP088922:2315473-2315754 [Rhodanobacter denitrificans]
MTIQSFCCKDTQALFERRRVRRFLVIEAAARRKLELLNAATQLDYLRVPPGNRLEALAGDRKGQHSIRVNDQFRLCFVWT
ADGPGLVEIVDYH

Nucleotide

• Length: 282 bp

>T227142 NZ_CP122724:c128623-128345 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCAAGCCAGGACAGAAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.79 Da
• Isoelectric Point: 7.9969

>AT227142 WP_007512782.1 NZ_CP088922:2315764-2316063 [Rhodanobacter denitrificans]
MPRKLPPIHPGEILREEYMLPLELSSNALARALEVTPARINEIVRERRGISADTALRLARYFGNDAQSWLNLQQRYDIEC
AQDSIGGALRKITPYQAAA

Nucleotide

• Length: 300 bp

>AT227142 NZ_CP122724:c128892-128623 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCGGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA