Detailed information of TA system TA227141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1130163..1130762
• Replicon: chromosome
• Accession: NZ_CP088922
• Organism: Rhodanobacter denitrificans strain FW104-10B01

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: LRK54_RS05275
• Protein ID: WP_027485489.1
• Coordinates: 1130481..1130762 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK54_RS05270
• Protein ID: WP_007510746.1
• Coordinates: 1130163..1130468 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK54_RS05250 (LRK54_05250)	1125618..1125848	-	231	WP_015446844.1	glutaredoxin family protein	-
LRK54_RS05255 (LRK54_05255)	1125924..1127306	+	1383	WP_027485492.1	L-serine ammonia-lyase	-
LRK54_RS05260 (LRK54_05260)	1127417..1127863	-	447	WP_027490858.1	hypothetical protein	-
LRK54_RS05265 (LRK54_05265)	1128076..1130025	+	1950	WP_231378428.1	site-specific DNA-methyltransferase	-
LRK54_RS05270 (LRK54_05270)	1130163..1130468	-	306	WP_007510746.1	HigA family addiction module antitoxin	Antitoxin
LRK54_RS05275 (LRK54_05275)	1130481..1130762	-	282	WP_027485489.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK54_RS05280 (LRK54_05280)	1130848..1133787	+	2940	WP_027490860.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (11-87)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10843.29 Da
• Isoelectric Point: 8.5334

>T227141 WP_027485489.1 NZ_CP088922:c1130762-1130481 [Rhodanobacter denitrificans]
VIRSFADKETARLSATGTSRRFPPDIVRRALSRLKYLHLATCPEDLREPFSNRLEMLSGNRAGQWSIRINDQWRLCFRFE
GGDAFDVEIVDYH

Nucleotide

• Length: 282 bp

>T227141 NZ_CP122724:c63386-63120 [Escherichia coli]
ATGGAGATATTTTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGT
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATGTCAGCGCCAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11196.00 Da
• Isoelectric Point: 7.3200

>AT227141 WP_007510746.1 NZ_CP088922:c1130468-1130163 [Rhodanobacter denitrificans]
MSIPATTNGLPPIHPGELLEEILSDHGMSQAAFARAIGVSPMRISHVIREERPVTAELALRFGRAFGQSPQFWMNMQTDY
DLKIAERELKASLRKVLPLAA

Nucleotide

• Length: 306 bp

>AT227141 NZ_CP122724:c63655-63386 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCTGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGTGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAGATGAGCAGGAAGATTGCCGGTAAATGA