Detailed information of TA system TA227138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3495886..3496476
• Replicon: chromosome
• Accession: NZ_CP088921
• Organism: Rhodanobacter denitrificans strain FW104-R3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: LRK44_RS16155
• Protein ID: WP_027490439.1
• Coordinates: 3496195..3496476 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK44_RS16150
• Protein ID: WP_007512782.1
• Coordinates: 3495886..3496185 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK44_RS16135 (LRK44_16135)	3491698..3492282	+	585	WP_027490441.1	hypothetical protein	-
LRK44_RS16140 (LRK44_16140)	3492369..3492533	-	165	Protein_3176	glycerophosphodiester phosphodiesterase family protein	-
LRK44_RS16145 (LRK44_16145)	3492596..3495832	-	3237	WP_027490440.1	type I restriction endonuclease subunit R	-
LRK44_RS16150 (LRK44_16150)	3495886..3496185	-	300	WP_007512782.1	HigA family addiction module antitoxin	Antitoxin
LRK44_RS16155 (LRK44_16155)	3496195..3496476	-	282	WP_027490439.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK44_RS16160 (LRK44_16160)	3496556..3497836	-	1281	WP_027490438.1	restriction endonuclease subunit S	-
LRK44_RS16165 (LRK44_16165)	3497833..3499908	-	2076	WP_027490437.1	class I SAM-dependent DNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10745.42 Da
• Isoelectric Point: 9.3345

>T227138 WP_027490439.1 NZ_CP088921:c3496476-3496195 [Rhodanobacter denitrificans]
MTIQSFCCKDTQALFERRRVRRFLVIEAAARRKLELLNAATQLDYLRVPPGNRLEALAGDRKGQHSIRVNDQFRLCFVWT
ADGPGLVEIVDYH

Nucleotide

• Length: 282 bp

>T227138 NZ_CP122722:c4752268-4752095 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.79 Da
• Isoelectric Point: 7.9969

>AT227138 WP_007512782.1 NZ_CP088921:c3496185-3495886 [Rhodanobacter denitrificans]
MPRKLPPIHPGEILREEYMLPLELSSNALARALEVTPARINEIVRERRGISADTALRLARYFGNDAQSWLNLQQRYDIEC
AQDSIGGALRKITPYQAAA

Nucleotide

• Length: 300 bp

>AT227138 NZ_CP122722:c4752592-4752296 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG