Detailed information of TA system TA227137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1392150..1392757
• Replicon: chromosome
• Accession: NZ_CP088921
• Organism: Rhodanobacter denitrificans strain FW104-R3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4WTS9
• Locus tag: LRK44_RS06340
• Protein ID: WP_007510671.1
• Coordinates: 1392150..1392464 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK44_RS06345
• Protein ID: WP_015447258.1
• Coordinates: 1392479..1392757 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK44_RS06335 (LRK44_06335)	1387947..1391678	+	3732	WP_027489851.1	diguanylate cyclase	-
LRK44_RS06340 (LRK44_06340)	1392150..1392464	+	315	WP_007510671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK44_RS06345 (LRK44_06345)	1392479..1392757	+	279	WP_015447258.1	helix-turn-helix domain-containing protein	Antitoxin
LRK44_RS06350 (LRK44_06350)	1392813..1393244	-	432	WP_027489850.1	Arc family DNA-binding protein	-
LRK44_RS06355 (LRK44_06355)	1393333..1393695	+	363	WP_015447260.1	hypothetical protein	-
LRK44_RS06360 (LRK44_06360)	1393760..1394524	+	765	WP_027489849.1	hypothetical protein	-
LRK44_RS06370 (LRK44_06370)	1394819..1395160	+	342	WP_007512800.1	lipid-A-disaccharide synthase N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11641.42 Da
• Isoelectric Point: 9.8349

>T227137 WP_007510671.1 NZ_CP088921:1392150-1392464 [Rhodanobacter denitrificans]
MIFVETPIFTAQIKDLLSDDDYGAFQRYLAENPEAGDVIKETGGLRKIRVAAKGHGKSGGARVIYYYFVSASRIAMVFAY
PKNVQENLTPAQKAALKKVIEQWR

Nucleotide

• Length: 315 bp

>T227137 NZ_CP122722:c4531453-4531079 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10263.75 Da
• Isoelectric Point: 7.2060

>AT227137 WP_015447258.1 NZ_CP088921:1392479-1392757 [Rhodanobacter denitrificans]
MFDNLMESVTQMDEIVRGERQPSREVHIDALAVKEIRRATGLSQAKFAGIVDVQVGTLRNWEQGRREPTGPAKALLRAIS
KDPKHVLEALAD

Nucleotide

• Length: 279 bp

>AT227137 NZ_CP122722:c4531911-4531543 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A154QK28