Detailed information of TA system TA227135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 722069..722668
• Replicon: chromosome
• Accession: NZ_CP088921
• Organism: Rhodanobacter denitrificans strain FW104-R3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: LRK44_RS03220
• Protein ID: WP_027485489.1
• Coordinates: 722069..722350 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK44_RS03225
• Protein ID: WP_007510746.1
• Coordinates: 722363..722668 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK44_RS03215 (LRK44_03215)	719044..721983	-	2940	WP_027490860.1	DEAD/DEAH box helicase family protein	-
LRK44_RS03220 (LRK44_03220)	722069..722350	+	282	WP_027485489.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK44_RS03225 (LRK44_03225)	722363..722668	+	306	WP_007510746.1	HigA family addiction module antitoxin	Antitoxin
LRK44_RS03230 (LRK44_03230)	722806..724755	-	1950	WP_231378428.1	site-specific DNA-methyltransferase	-
LRK44_RS03235 (LRK44_03235)	724968..725414	+	447	WP_027490858.1	hypothetical protein	-
LRK44_RS03240 (LRK44_03240)	725525..726907	-	1383	WP_027485492.1	L-serine ammonia-lyase	-
LRK44_RS03245 (LRK44_03245)	726983..727213	+	231	WP_015446844.1	glutaredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (11-87)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10843.29 Da
• Isoelectric Point: 8.5334

>T227135 WP_027485489.1 NZ_CP088921:722069-722350 [Rhodanobacter denitrificans]
VIRSFADKETARLSATGTSRRFPPDIVRRALSRLKYLHLATCPEDLREPFSNRLEMLSGNRAGQWSIRINDQWRLCFRFE
GGDAFDVEIVDYH

Nucleotide

• Length: 282 bp

>T227135 NZ_CP122722:3915125-3915343 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11196.00 Da
• Isoelectric Point: 7.3200

>AT227135 WP_007510746.1 NZ_CP088921:722363-722668 [Rhodanobacter denitrificans]
MSIPATTNGLPPIHPGELLEEILSDHGMSQAAFARAIGVSPMRISHVIREERPVTAELALRFGRAFGQSPQFWMNMQTDY
DLKIAERELKASLRKVLPLAA

Nucleotide

• Length: 306 bp

>AT227135 NZ_CP122722:3914725-3915099 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG