Detailed information of TA system TA227134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3495890..3496480
• Replicon: chromosome
• Accession: NZ_CP088920
• Organism: Rhodanobacter denitrificans strain FW104-10F02

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: LRK32_RS16145
• Protein ID: WP_027490439.1
• Coordinates: 3496199..3496480 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRK32_RS16140
• Protein ID: WP_007512782.1
• Coordinates: 3495890..3496189 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRK32_RS16125 (LRK32_16125)	3491702..3492286	+	585	WP_027490441.1	hypothetical protein	-
LRK32_RS16130 (LRK32_16130)	3492373..3492537	-	165	Protein_3174	glycerophosphodiester phosphodiesterase family protein	-
LRK32_RS16135 (LRK32_16135)	3492600..3495836	-	3237	WP_027490440.1	type I restriction endonuclease subunit R	-
LRK32_RS16140 (LRK32_16140)	3495890..3496189	-	300	WP_007512782.1	HigA family addiction module antitoxin	Antitoxin
LRK32_RS16145 (LRK32_16145)	3496199..3496480	-	282	WP_027490439.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRK32_RS16150 (LRK32_16150)	3496560..3497840	-	1281	WP_027490438.1	restriction endonuclease subunit S	-
LRK32_RS16155 (LRK32_16155)	3497837..3499912	-	2076	WP_027490437.1	class I SAM-dependent DNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10745.42 Da
• Isoelectric Point: 9.3345

>T227134 WP_027490439.1 NZ_CP088920:c3496480-3496199 [Rhodanobacter denitrificans]
MTIQSFCCKDTQALFERRRVRRFLVIEAAARRKLELLNAATQLDYLRVPPGNRLEALAGDRKGQHSIRVNDQFRLCFVWT
ADGPGLVEIVDYH

Nucleotide

• Length: 282 bp

>T227134 NZ_CP122722:c3402856-3402479 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.79 Da
• Isoelectric Point: 7.9969

>AT227134 WP_007512782.1 NZ_CP088920:c3496189-3495890 [Rhodanobacter denitrificans]
MPRKLPPIHPGEILREEYMLPLELSSNALARALEVTPARINEIVRERRGISADTALRLARYFGNDAQSWLNLQQRYDIEC
AQDSIGGALRKITPYQAAA

Nucleotide

• Length: 300 bp

>AT227134 NZ_CP122722:c3403313-3402945 [Escherichia coli]
GTGTCAGACTCACTCCATGAAACAAATTATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCCTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA