Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 722074..722673 Replicon chromosome
Accession NZ_CP088920
Organism Rhodanobacter denitrificans strain FW104-10F02

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag LRK32_RS03215 Protein ID WP_027485489.1
Coordinates 722074..722355 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LRK32_RS03220 Protein ID WP_007510746.1
Coordinates 722368..722673 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRK32_RS03210 (LRK32_03210) 719049..721988 - 2940 WP_027490860.1 DEAD/DEAH box helicase family protein -
LRK32_RS03215 (LRK32_03215) 722074..722355 + 282 WP_027485489.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LRK32_RS03220 (LRK32_03220) 722368..722673 + 306 WP_007510746.1 HigA family addiction module antitoxin Antitoxin
LRK32_RS03225 (LRK32_03225) 722811..724760 - 1950 WP_231378428.1 site-specific DNA-methyltransferase -
LRK32_RS03230 (LRK32_03230) 724973..725419 + 447 WP_027490858.1 hypothetical protein -
LRK32_RS03235 (LRK32_03235) 725530..726912 - 1383 WP_027485492.1 L-serine ammonia-lyase -
LRK32_RS03240 (LRK32_03240) 726988..727218 + 231 WP_015446844.1 glutaredoxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(11-87)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10843.29 Da        Isoelectric Point: 8.5334

>T227131 WP_027485489.1 NZ_CP088920:722074-722355 [Rhodanobacter denitrificans]
VIRSFADKETARLSATGTSRRFPPDIVRRALSRLKYLHLATCPEDLREPFSNRLEMLSGNRAGQWSIRINDQWRLCFRFE
GGDAFDVEIVDYH

Download         Length: 282 bp

>T227131 NZ_CP122722:c2550250-2548928 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGATGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11196.00 Da        Isoelectric Point: 7.3200

>AT227131 WP_007510746.1 NZ_CP088920:722368-722673 [Rhodanobacter denitrificans]
MSIPATTNGLPPIHPGELLEEILSDHGMSQAAFARAIGVSPMRISHVIREERPVTAELALRFGRAFGQSPQFWMNMQTDY
DLKIAERELKASLRKVLPLAA

Download         Length: 306 bp

>AT227131 NZ_CP122722:c2550516-2550250 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References