Detailed information of TA system TA022713

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40255..40681
• Replicon: plasmid pSMS35_130
• Accession: NC_010488
• Organism: Escherichia coli SMS-3-5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECSMS35_RS25165
• Protein ID: WP_001312861.1
• Coordinates: 40255..40413 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40457..40681 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSMS35_RS25130	35300..35527	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
ECSMS35_RS25135	35621..36307	-	687	WP_000332484.1	PAS domain-containing protein	-
ECSMS35_RS25140	36498..36881	-	384	WP_000124981.1	relaxosome protein TraM	-
ECSMS35_RS25145	37158..37805	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
ECSMS35_RS25150	38102..38923	-	822	WP_001234469.1	DUF945 domain-containing protein	-
ECSMS35_RS25155	39046..39333	-	288	WP_000107535.1	hypothetical protein	-
ECSMS35_RS28535	39358..39564	-	207	WP_000547939.1	hypothetical protein	-
ECSMS35_RS25165	40255..40413	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	40457..40681	-	225	NuclAT_0	-	Antitoxin
-	40457..40681	-	225	NuclAT_0	-	Antitoxin
-	40457..40681	-	225	NuclAT_0	-	Antitoxin
-	40457..40681	-	225	NuclAT_0	-	Antitoxin
ECSMS35_RS29575	40493..40681	+	189	WP_001299721.1	hypothetical protein	-
ECSMS35_RS25175	40693..41412	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
ECSMS35_RS25180	41409..41843	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
ECSMS35_RS25185	41898..43856	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
ECSMS35_RS25190	43915..44148	-	234	WP_000006004.1	DUF905 domain-containing protein	-
ECSMS35_RS25195	44204..44731	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
ECSMS35_RS28545	45204..45470	-	267	WP_072254135.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / catA2 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aadA2 / tet(A) / aph(3')-Ia	-	1..130440	130440

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T22713 WP_001312861.1 NC_010488:c40413-40255 [Escherichia coli SMS-3-5]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T22713 NC_010488:c40413-40255 [Escherichia coli SMS-3-5]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT22713 NC_010488:c40681-40457 [Escherichia coli SMS-3-5]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin