Detailed information of TA system TA227126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3177153..3177743
• Replicon: chromosome
• Accession: NZ_CP088918
• Organism: Rhodanobacter denitrificans strain FW107-2APBS1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: I4WLY1
• Locus tag: LRJ86_RS14920
• Protein ID: WP_007512781.1
• Coordinates: 3177462..3177743 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRJ86_RS14915
• Protein ID: WP_007512782.1
• Coordinates: 3177153..3177452 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRJ86_RS14900 (LRJ86_14900)	3172965..3173549	+	585	WP_015448948.1	hypothetical protein	-
LRJ86_RS14905 (LRJ86_14905)	3173636..3173800	-	165	Protein_2932	glycerophosphodiester phosphodiesterase family protein	-
LRJ86_RS14910 (LRJ86_14910)	3173863..3177099	-	3237	WP_015448949.1	type I restriction endonuclease subunit R	-
LRJ86_RS14915 (LRJ86_14915)	3177153..3177452	-	300	WP_007512782.1	HigA family addiction module antitoxin	Antitoxin
LRJ86_RS14920 (LRJ86_14920)	3177462..3177743	-	282	WP_007512781.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRJ86_RS14925 (LRJ86_14925)	3177823..3179076	-	1254	WP_015448950.1	restriction endonuclease subunit S	-
LRJ86_RS14930 (LRJ86_14930)	3179073..3181046	-	1974	WP_007512779.1	class I SAM-dependent DNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10798.46 Da
• Isoelectric Point: 10.3017

>T227126 WP_007512781.1 NZ_CP088918:c3177743-3177462 [Rhodanobacter denitrificans]
MTIQSFRCKDTQALFERRRVRRFLVIEAAARRKLELLNAATQLDYLRVPPGNRLEALAGDRKGQHSIRVNDQFRLCFVWT
ADGPGLVEIVDYH

Nucleotide

• Length: 282 bp

>T227126 NZ_CP122722:1954514-1954768 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.79 Da
• Isoelectric Point: 7.9969

>AT227126 WP_007512782.1 NZ_CP088918:c3177452-3177153 [Rhodanobacter denitrificans]
MPRKLPPIHPGEILREEYMLPLELSSNALARALEVTPARINEIVRERRGISADTALRLARYFGNDAQSWLNLQQRYDIEC
AQDSIGGALRKITPYQAAA

Nucleotide

• Length: 300 bp

>AT227126 NZ_CP122722:1954266-1954517 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: I4WLY1