Detailed information of TA system TA227125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 943817..944424
• Replicon: chromosome
• Accession: NZ_CP088918
• Organism: Rhodanobacter denitrificans strain FW107-2APBS1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4WTS9
• Locus tag: LRJ86_RS04350
• Protein ID: WP_007510671.1
• Coordinates: 943817..944131 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRJ86_RS04355
• Protein ID: WP_015447258.1
• Coordinates: 944146..944424 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRJ86_RS04345 (LRJ86_04345)	939614..943345	+	3732	WP_007510668.1	diguanylate cyclase	-
LRJ86_RS04350 (LRJ86_04350)	943817..944131	+	315	WP_007510671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRJ86_RS04355 (LRJ86_04355)	944146..944424	+	279	WP_015447258.1	helix-turn-helix domain-containing protein	Antitoxin
LRJ86_RS04360 (LRJ86_04360)	944480..944911	-	432	WP_027489850.1	Arc family DNA-binding protein	-
LRJ86_RS04365 (LRJ86_04365)	945000..945362	+	363	WP_015447260.1	hypothetical protein	-
LRJ86_RS04370 (LRJ86_04370)	945427..946191	+	765	WP_015447261.1	hypothetical protein	-
LRJ86_RS04380 (LRJ86_04380)	946486..946827	+	342	WP_007512800.1	lipid-A-disaccharide synthase N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11641.42 Da
• Isoelectric Point: 9.8349

>T227125 WP_007510671.1 NZ_CP088918:943817-944131 [Rhodanobacter denitrificans]
MIFVETPIFTAQIKDLLSDDDYGAFQRYLAENPEAGDVIKETGGLRKIRVAAKGHGKSGGARVIYYYFVSASRIAMVFAY
PKNVQENLTPAQKAALKKVIEQWR

Nucleotide

• Length: 315 bp

>T227125 NZ_CP122722:1954266-1954517 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10263.75 Da
• Isoelectric Point: 7.2060

>AT227125 WP_015447258.1 NZ_CP088918:944146-944424 [Rhodanobacter denitrificans]
MFDNLMESVTQMDEIVRGERQPSREVHIDALAVKEIRRATGLSQAKFAGIVDVQVGTLRNWEQGRREPTGPAKALLRAIS
KDPKHVLEALAD

Nucleotide

• Length: 279 bp

>AT227125 NZ_CP122722:1954514-1954768 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A154QK28