Detailed information of TA system TA227124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 276123..276722
• Replicon: chromosome
• Accession: NZ_CP088918
• Organism: Rhodanobacter denitrificans strain FW107-2APBS1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: I4WT83
• Locus tag: LRJ86_RS01255
• Protein ID: WP_007510748.1
• Coordinates: 276123..276404 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRJ86_RS01260
• Protein ID: WP_007510746.1
• Coordinates: 276417..276722 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRJ86_RS01250 (LRJ86_01250)	273098..276037	-	2940	WP_015446840.1	DEAD/DEAH box helicase family protein	-
LRJ86_RS01255 (LRJ86_01255)	276123..276404	+	282	WP_007510748.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRJ86_RS01260 (LRJ86_01260)	276417..276722	+	306	WP_007510746.1	HigA family addiction module antitoxin	Antitoxin
LRJ86_RS01265 (LRJ86_01265)	276860..278803	-	1944	WP_041676852.1	DNA methyltransferase	-
LRJ86_RS01270 (LRJ86_01270)	278896..279324	+	429	WP_015446842.1	hypothetical protein	-
LRJ86_RS01275 (LRJ86_01275)	279453..280835	-	1383	WP_015446843.1	L-serine ammonia-lyase	-
LRJ86_RS01280 (LRJ86_01280)	280911..281141	+	231	WP_015446844.1	glutaredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (11-87)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10873.31 Da
• Isoelectric Point: 7.8618

>T227124 WP_007510748.1 NZ_CP088918:276123-276404 [Rhodanobacter denitrificans]
VIRSFADKETARLSATGTSRRFPPDIVRWALSRLKYLHLATCPEDLREPFSNRLEMLSGNRAGQWSIRINDQWRLCFRFE
GGDAFDVEIVDYH

Nucleotide

• Length: 282 bp

>T227124 NZ_CP122722:1461665-1462063 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11196.00 Da
• Isoelectric Point: 7.3200

>AT227124 WP_007510746.1 NZ_CP088918:276417-276722 [Rhodanobacter denitrificans]
MSIPATTNGLPPIHPGELLEEILSDHGMSQAAFARAIGVSPMRISHVIREERPVTAELALRFGRAFGQSPQFWMNMQTDY
DLKIAERELKASLRKVLPLAA

Nucleotide

• Length: 306 bp

>AT227124 NZ_CP122722:1461438-1461665 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: I4WT83