Detailed information of TA system TA227122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 362706..363325
• Replicon: plasmid pLP1
• Accession: NZ_CP088909
• Organism: Rhodococcus qingshengii strain VT6

Toxin (Protein)

• Gene name: graT
• Uniprot ID: C3JNS8
• Locus tag: LRL17_RS32860
• Protein ID: WP_003943404.1
• Coordinates: 363044..363325 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRL17_RS32855
• Protein ID: WP_003943405.1
• Coordinates: 362706..362999 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRL17_RS32830 (LRL17_32830)	358747..359841	-	1095	Protein_355	hypothetical protein	-
LRL17_RS32835 (LRL17_32835)	359958..360293	+	336	WP_178377381.1	hypothetical protein	-
LRL17_RS32840 (LRL17_32840)	360479..360961	+	483	Protein_357	serine/threonine protein kinase	-
LRL17_RS32845 (LRL17_32845)	361027..361386	-	360	WP_166814174.1	hypothetical protein	-
LRL17_RS32850 (LRL17_32850)	361615..362604	+	990	WP_005569266.1	recombinase family protein	-
LRL17_RS32855 (LRL17_32855)	362706..362999	-	294	WP_003943405.1	HigA family addiction module antitoxin	Antitoxin
LRL17_RS32860 (LRL17_32860)	363044..363325	-	282	WP_003943404.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRL17_RS32865 (LRL17_32865)	363490..365835	-	2346	WP_073513716.1	TniQ family protein	-
LRL17_RS32870 (LRL17_32870)	365832..366932	-	1101	WP_003945389.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..501672	501672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-81)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11011.53 Da
• Isoelectric Point: 9.5432

>T227122 WP_003943404.1 NZ_CP088909:c363325-363044 [Rhodococcus qingshengii]
VIESFADKETERVFRRQFSKKLPQDIQRIARRRLLLIEAATDVNDLRVPPGNRLEKLSGDRVGQYSVRINDQYRICFIWR
ATAAYDVEITDYH

Nucleotide

• Length: 282 bp

>T227122 NZ_CP122722:1086063-1086398 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10799.40 Da
• Isoelectric Point: 7.2035

>AT227122 WP_003943405.1 NZ_CP088909:c362999-362706 [Rhodococcus qingshengii]
MTTDLSPIHPGEILREDFLEPFGITQNRLATIIGVPPRRINEIVHGKRSITADTALRLAKAFGNSAQFWLNIQDRYELDL
AADKLGDTLAKITRLSA

Nucleotide

• Length: 294 bp

>AT227122 NZ_CP122722:1085815-1086063 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7Z6WKI4