Detailed information of TA system TA227114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4665710..4666312
• Replicon: chromosome
• Accession: NZ_CP088901
• Organism: Salmonella enterica subsp. enterica serovar Muenchen strain 180135033

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: LRP35_RS22800
• Protein ID: WP_001159630.1
• Coordinates: 4666001..4666312 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRP35_RS22795
• Protein ID: WP_000362050.1
• Coordinates: 4665710..4666000 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRP35_RS22780 (4663203)	4663203..4664105	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LRP35_RS22785 (4664102)	4664102..4664737	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LRP35_RS22790 (4664734)	4664734..4665663	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LRP35_RS22795 (4665710)	4665710..4666000	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
LRP35_RS22800 (4666001)	4666001..4666312	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
LRP35_RS22805 (4666530)	4666530..4667459	+	930	WP_021294279.1	alpha/beta hydrolase	-
LRP35_RS22810 (4667545)	4667545..4667856	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
LRP35_RS22815 (4667853)	4667853..4668299	+	447	WP_001259012.1	type II toxin-antitoxin system HigA family antitoxin	-
LRP35_RS22820 (4668314)	4668314..4669255	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LRP35_RS22825 (4669300)	4669300..4669737	-	438	WP_001621365.1	D-aminoacyl-tRNA deacylase	-
LRP35_RS22830 (4669734)	4669734..4670606	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LRP35_RS22835 (4670600)	4670600..4671199	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T227114 WP_001159630.1 NZ_CP088901:c4666312-4666001 [Salmonella enterica subsp. enterica serovar Muenchen]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T227114 NZ_CP122719:c4879309-4878998 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT227114 WP_000362050.1 NZ_CP088901:c4666000-4665710 [Salmonella enterica subsp. enterica serovar Muenchen]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT227114 NZ_CP122719:c4878997-4878707 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0R9NS64